Possible sources of dye-related signal correlation bias in two-color DNA microarray assays.
Authors:Cox WG, Beaudet MP, Agnew JY, Ruth JL
Journal:Anal Biochem
PubMed ID:15265729
'DNA microarray analyses commonly use two spectrally distinct fluorescent labels to simultaneously compare different mRNA pools. Signal correlation bias currently limits accepted resolution to twofold changes in gene expression. This bias was investigated by (i) examining fluorescence and absorption spectra and changes in relative fluorescence of DNAs labeled with the ... More
Effects of atmospheric ozone on microarray data quality.
Authors:Fare TL, Coffey EM, Dai H, He YD, Kessler DA, Kilian KA, Koch JE, LeProust E, Marton MJ, Meyer MR, Stoughton RB, Tokiwa GY, Wang Y
Journal:Anal Chem
PubMed ID:14632079
A data anomaly was observed that affected the uniformity and reproducibility of fluorescent signal across DNA microarrays. Results from experimental sets designed to identify potential causes (from microarray production to array scanning) indicated that the anomaly was linked to a batch process; further work allowed us to localize the effect ... More
Exploring the regulation of tRNA distribution on the genomic scale.
Authors:Dittmar KA, Mobley EM, Radek AJ, Pan T
Journal:J Mol Biol
PubMed ID:15001350
Though up to 20% of the total RNA in bacterial cells is tRNA, the regulation of tRNA distribution on the genomic level remains unclear. tRNA distribution is governed by four processes: transcription, processing of precursor tRNA, degradation of precursor tRNA and degradation of mature tRNA. To elucidate the relationship between ... More