LV-MAX™ Production Medium
LV-MAX™ Production Medium
Gibco™

LV-MAX™ Production Medium

Gibco LV-MAX産生培地は、化学的に定義された血清を含まず、タンパク質を含まない培地で、Gibcoウイルス産生細胞(懸濁液に適合したHEK 293由来細胞)の高密度成長およびトランスフェクション用に特別に開発され、高力価のレンチウイルス産生を促進します。•1.0詳細を見る
製品番号(カタログ番号)数量
A35834011 L
A35834026 x 1 L
製品番号(カタログ番号) A3583401
価格(JPY)
35,700
Each
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数量:
1 L
Gibco LV-MAX産生培地は、化学的に定義された血清を含まず、タンパク質を含まない培地で、Gibcoウイルス産生細胞(懸濁液に適合したHEK 293由来細胞)の高密度成長およびトランスフェクション用に特別に開発され、高力価のレンチウイルス産生を促進します。

•1.0 x 107細胞/mLを超える密度までのウイルス産生細胞の成長をサポートします
• 高密度の一時的トランスフェクション培養を持続的かつ高レベルで産生でき、最大1 x 108 TU/mL(非濃縮LVVS-GFP)の力価を達成します
• 96-ディープウェルブロックから2-Lのフラスコおよびバイオリアクターまでの培養フォーマットでのウイルス産生細胞の成長およびトランスフェクションをサポートします

LV-MAC産出培地はすぐに使用でき、添加は不要です。化学的に定義された組成により、高い再現性とロット間での信頼性が得られます。この培地は接着性細胞培養には推奨されません。

研究用にのみ使用できます。診断用には使用いただけません。
仕様
細胞株Viral Production Cells
細胞タイプHEK 293
抗生物質を含むNo antibiotics
製造品質ISO 13485, MDSAP, FDA-registered
製品ラインLV-MAX
製品タイプLentiviral Production Medium
数量1 L
品質保持期間12 Months
出荷条件Room Temperature
分類Animal Origin-free, Chemically-defined, Protein-free, Serum-free
Culture TypeSuspension Cell Culture
形状Liquid
無菌性Sterile-filtered
添加剤ありGlutaMAX
Unit SizeEach
組成および保存条件
1 L bottle; Reagents should be stored at 2°C to 8°C, protected from light

よくあるご質問(FAQ)

What are the major differences between LV-MAX and CTS LV- MAX products?

The LV-MAX and CTS LV- MAX products have equivalent performance. In contrast to the research use only LV-MAX products, CTS LV-MAX products have an additional intended use for cell and gene therapy applications, and are complemented with a more robust regulatory support package to enable clinical therapeutic applications. This means that to the best of our ability, we keep our CTS product specifications and release requirements up-to-date in accordance with the latest regulatory guidance specific for this intended use.

What is the intended use claim for your LV-MAX and CTS LV-MAX products?

Our LV-MAX products are intended for research use only while our CTS LV- MAX products are intended for research use or manufacture of cell, gene or tissue based products. Please refer to the individual products for further details on the intended use claim.

Do you offer a lentivirus production service?

Yes. Please provide your details here (https://www.thermofisher.com/us/en/home/global/forms/life-science/lentiviral-vector-production-get-info.html) and mention your interest in our lentivirus production services. A member of our services team will contact you shortly.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

With the LV-MAX Production System, are the lentiviral particles stable in packaging media?

Lentiviral particles packaged in our LV-MAX Production Medium will be stable at 4 degrees C for 24 hours and will then begin to lose activity. For long-term storage, we recommend storing at –80 degrees C.

With the LV-MAX Production System, can I use cell lines besides HT1080 for titering my lentivirus?

We strongly recommend the human fibrosarcoma line HT1080 (ATCC, Cat. No. CCL-121) as the “gold standard” for reproducibly titering lentivirus. However, you may wish to use the same mammalian cell line to titer your lentiviral stocks as you will use to perform your expression studies (e.g., if you are performing expression studies in a dividing cell line or a non-primary cell line). If you have more than one lentiviral construct, we recommend that you titer all of the lentiviral constructs using the same mammalian cell line.