Amplex™ Red and Amplex™ UltraRed Reagents
Amplex™ Red and Amplex™ UltraRed Reagents
Citations & References (15)
Invitrogen™

Amplex™ Red and Amplex™ UltraRed Reagents

H2O2用の高感度で安定したプローブであるAmplex Red試薬は、ペルオキシダーゼに最適な蛍光基質です。西洋ワサビペルオキシダーゼ（HRP）が存在する場合、Amplex Red試薬はH2O2と1：1の化学量論比で反応し、高蛍光性のレゾルフィンを産生しますH2O2は多くの異なる酵素反応で生成されるため、Amplex詳細を見る
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製品番号（カタログ番号）数量染色剤タイプ
A360065 x 1 mgAmplex UltraRed Reagent
A122225 mgAmplex Red Reagent
A2217710 x 10 mgAmplex Red Reagent
製品番号（カタログ番号） A36006
価格（JPY）
64,300
Each
お問い合わせください ›
数量:
5 x 1 mg
染色剤タイプ:
Amplex UltraRed Reagent
H2O2用の高感度で安定したプローブであるAmplex Red試薬は、ペルオキシダーゼに最適な蛍光基質です。西洋ワサビペルオキシダーゼ（HRP）が存在する場合、Amplex Red試薬はH2O2と1：1の化学量論比で反応し、高蛍光性のレゾルフィンを産生しますH2O2は多くの異なる酵素反応で生成されるため、Amplex Red試薬を使用して多くの異なる酵素の活性を検出できます。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
検出法蛍光
染色剤タイプAmplex UltraRed Reagent
形状固体
数量5 x 1 mg
出荷条件室温
基質HRP (Horseradish Peroxidase) Substrate
基質タイプChemical substrate
検証済みアプリケーションCell Analysis
使用対象（アプリケーション）Hydrogen Peroxidase Assay
使用対象 (装置)Fluorometer, Microplate Reader
製品ラインAmplex
製品タイプRed Hydrogen Peroxide
Unit SizeEach
組成および保存条件
冷凍庫（-5～-30℃）に保存。

よくあるご質問（FAQ）

I'm using an Amplex Red kit, the reagent changes color to pink almost immediately in my own Krebs-Ringer buffer but not in HBSS. Why is this?

The components of Krebs-Ringer buffer (salts) should not cause oxidation of the Amplex reagent (which, in the presence of peroxidase and H2O2 oxidizes to resorufin, which is pink in color and fluorescent). Try water alone (the water used to make the Krebs-Ringer buffer). Since Hank's Buffered Saline Solution is typically purchased rather than made in the lab, it likely would not have the same contaminant. Another option is to degas the buffer prior to use to removed dissolved oxygen radicals.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (15)

引用および参考文献
Abstract
A genetically encoded photosensitizer.
Authors:Bulina ME, Chudakov DM, Britanova OV, Yanushevich YG, Staroverov DB, Chepurnykh TV, Merzlyak EM, Shkrob MA, Lukyanov S, Lukyanov KA
Journal:Nat Biotechnol
PubMed ID:16369538
'Photosensitizers are chromophores that generate reactive oxygen species (ROS) upon light irradiation. They are used for inactivation of specific proteins by chromophore-assisted light inactivation (CALI) and for light-induced cell killing in photodynamic therapy. Here we report a genetically encoded photosensitizer, which we call KillerRed, developed from the hydrozoan chromoprotein anm2CP, ... More
Amplex UltraRed enhances the sensitivity of fluorimetric pyruvate detection.
Authors:Zhu A, Romero R, Petty HR,
Journal:Anal Biochem
PubMed ID:20382105
Pyruvate, which is produced in the final step of glycolysis, participates in anabolic metabolism, catabolic metabolism, and signal transduction. We have recently reported a new sensitive and selective fluorimetric assay for pyruvate measurement using Amplex Red as a fluorogenic dye. However, the fluorescence of the reaction product, resorufin, is pH ... More
Ultrahigh-throughput screening in drop-based microfluidics for directed evolution.
Authors:Agresti JJ, Antipov E, Abate AR, Ahn K, Rowat AC, Baret JC, Marquez M, Klibanov AM, Griffiths AD, Weitz DA,
Journal:Proc Natl Acad Sci U S A
PubMed ID:20142500
The explosive growth in our knowledge of genomes, proteomes, and metabolomes is driving ever-increasing fundamental understanding of the biochemistry of life, enabling qualitatively new studies of complex biological systems and their evolution. This knowledge also drives modern biotechnologies, such as molecular engineering and synthetic biology, which have enormous potential to ... More
Resveratrol promotes clearance of Alzheimer's disease amyloid-beta peptides.
Authors:Marambaud P, Zhao H, Davies P,
Journal:J Biol Chem
PubMed ID:16162502
Several epidemiological studies indicate that moderate consumption of wine is associated with a lower incidence of Alzheimer's disease. Wine is enriched in antioxidant compounds with potential neuroprotective activities. However, the exact molecular mechanisms involved in the beneficial effects of wine intake on the neurodegenerative process in Alzheimer's disease brain remain ... More
Facile SNP detection using bifunctional, cross-linking oligonucleotide probes.
Authors:Peng X, Greenberg MM,
Journal:Nucleic Acids Res
PubMed ID:18281702
A facile, sensitive method for detecting specific sequences of oligonucleotides was developed. Detection of DNA sequences with single nucleotide discrimination is achieved by combining the selectivity of hybridization with an efficient cross-linking reaction. Readily synthesized bifunctional oligonucleotide probes containing a modified pyrimidine that is capable of forming interstrand cross-links under ... More
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