Primary Rat Cortex Neurons, Sprague Dawley
Primary Rat Cortex Neurons, Sprague Dawley
Gibco™

Primary Rat Cortex Neurons, Sprague Dawley

Gibco Primary Rat Cortex Neurons、Sprague Dawleyは、18日目のSprague Dawleyラット胚から単離し、凍結保存されています詳細を見る
製品番号(カタログ番号)セル数
A365124 x 10^6
A365111 x 10^6
製品番号(カタログ番号) A36512
価格(JPY)
89,100
Each
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セル数:
4 x 10^6

Gibco Primary Rat Cortex Neurons、Sprague Dawleyは、18日目のSprague Dawleyラット胚から単離し、凍結保存されています。新鮮なニューロンに代わる柔軟ですぐに使用可能な高品質の代替品で、各バイアルには1 mLあたり100万個の細胞が含まれています。これらのニューロンは一貫して50~80%の範囲の解凍後生存率を示し、高い神経細胞純度を有し、機能性が検証されています。

高い生存率:解凍後生存率が50%以上
高純度:70%を超える神経細胞表現型
機能検証済み:自発的同調カルシウム流動およびシナプス放出活性について試験済み
 

研究用途にのみご使用ください。診断目的には使用できません。
仕様
フォーマットOne vial containing 4 million cells
セル数4 x 10^6
製品タイプラット皮質ニューロン
数量4 x 106 cells
試験済み細胞の純度、無菌性、生存率、マイコプラズマ
Age胎児
細胞タイプ神経細胞
Culture Type哺乳類細胞培養
形状凍結保存済み
ラット
Tissue皮質
Unit SizeEach
組成および保存条件
液体窒素で保存

よくあるご質問(FAQ)

What is the difference between Primary Rat Cortex Neurons, Sprague Dawley and Primary Rat Cortex Neurons?

These neurons differ in the rat strain from which they were isolated: Sprague Dawley vs. Fisher 344. These neurons don't expand anymore. They just grow neurites. The manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0001574-PrimaryRatCortex-HippocampusNeurons-UG.pdf) has instructions for culturing the cells. You can use Neurobasal Plus/B-27 Plus/GlutaMAX to culture them, or just Neurobasal/B-27/GlutaMAX. However, the former combination provides much superior performance than the latter and can maintain neurons longer.

I'm currently culturing neurons. Which media system do you recommend using in conjunction with the Poly-D-Lysine matrix?

We recommend using the B-27 Plus Neuronal Culture System when culturing neurons on Poly-D-Lysine. The B-27 Plus Neuronal Culture System is a complete media system that includes the B-27 Plus Supplement and the Neurobasal Plus Medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What cell types will Poly-D-Lysine support as an attachment matrix?

The Poly-D-Lysine matrix has successfully supported the attachment of primary rodent neurons, iPSC-derived neurons and PC12 cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can primary rat neurons be cryopreserved again after they are cultured?

Primary rat neurons cannot be cryopreserved again because they will not revive.

Can primary rat neurons proliferate?

No, primary rat neurons do not proliferate.