I'm currently culturing neurons. Which media system do you recommend using in conjunction with the Poly-D-Lysine matrix?
We recommend using the B-27 Plus Neuronal Culture System when culturing neurons on Poly-D-Lysine. The B-27 Plus Neuronal Culture System is a complete media system that includes the B-27 Plus Supplement and the Neurobasal Plus Medium.
What is the purpose of L-glutamate in the media for primary hippocampal neurons?
Glutamate helps hippocampal neurons sprout and promotes viability. However, L-glutamate can only be supplemented in the medium up to day 4 in culture because it is toxic to mature neurons.
Can primary rat neurons be cryopreserved again after they are cultured?
Primary rat neurons cannot be cryopreserved again because they will not revive.