Tandem Mass Tag(TMT)試薬は、タンデム質量分析を使用してさまざまなサンプル中のタンパク質を同定および定量できるように特別に設計されています。TMT11-131Cタグの構造は他のTMT10pexタグと同じですが、TMT11-131C質量レポーターは13C同位体のみで標識されています。TMT11-131Cタグを付加することで、元のTMT10plex試薬の131質量レポーターイオンをNおよびCバリアントに分離し、高分解能Orbitrap MS装置を使用した相対定量用に11番目のチャンネルを作成できます。
TMT11-131Cは、スタンドアロンタグとして、またはTMT10plex Isobaric Label Reagent Set plus TMT11-131C(カタログ番号A34808)の一部として購入できます。TMT試薬は業界最先端の高分解能Orbitrap装置およびソフトウェアと組み合わせることで、タンパク質発現の定量分析において包括的ソリューションを提供します。
For Research Use Only. Not for use in diagnostic procedures.
仕様
最終産物タイプPeptide
使用対象 (装置)質量分析計
標識または色素Mass Isotopes of Amino Acids
数量3 x 0.8 mg (per tag)
出荷条件Dry Ice
ワークフローステップPeptide Labeling
検出法Mass Spectrometry
製品ラインTMT10plex
製品タイプIsobaric Label Reagent
原料Peptides
Unit SizeEach
組成および保存条件
3 sets of 11 vials, store at –5 to –30°C
引用および参考文献 (4)
引用および参考文献
Abstract
Cell-type and subcellular compartment-specific APEX2 proximity labeling reveals activity-dependent nuclear proteome dynamics in the striatum.
Authors:Dumrongprechachan V,Salisbury RB,Soto G,Kumar M,MacDonald ML,Kozorovitskiy Y
Journal:Nature communications
PubMed ID:34381044
The vertebrate brain consists of diverse neuronal types, classified by distinct anatomy and function, along with divergent transcriptomes and proteomes. Defining the cell-type specific neuroproteomes is important for understanding the development and functional organization of neural circuits. This task remains challenging in complex tissue, due to suboptimal protein isolation techniques ... More
Comparison of plastid proteomes points towards a higher plastidial redox turnover in vascular tissues than in mesophyll cells.
Authors:Boussardon C,Carrie C,Keech O
Journal:Journal of experimental botany
PubMed ID:37026385
Plastids are complex organelles that vary in size and function depending on the cell type. Accordingly, they can be referred to as amyloplasts, chloroplasts, chromoplasts, etioplasts, or proplasts, to only cite a few. Over the past decades, methods based on density gradients and differential centrifugation have been extensively used for ... More
Correction: Biochemical characterization of protease activity of Nsp3 from SARS-CoV-2 and its inhibition by nanobodies
Authors:Armstrong LA, Lange SM, Cesare V, Matthews SP, Nirujogi RS, Cole I, Hope A, Cunningham F, Toth R, Mukherjee R, Bojkova D, Gruber F, Gray D, Wyatt PG, Cinatl J, Dikic I, Davies P, Kulathu Y.
Decellularization alters the unfavorable regenerative adverse microenvironment of the injured spinal cord to support neurite outgrowth.
Authors:Hu J,Shangguan J,Askar P,Xu J,Sun H,Zhou S,Zhu C,Su W,Gu Y
Journal:Annals of translational medicine
PubMed ID:36172103
BACKGROUND: Acellular tissue has been transplanted into the injury site as an external microenvironment to intervene with imbalance microenvironment that occurs after spinal cord injury (SCI) and stimulating axonal regeneration, although the mechanism is unclear. Given decellularization is the key means to obtain acellular tissues, we speculated changes in the ... More