TaqMan Universal DNA Spike-In Control serves as an exogenous process control of a known concentration that is spiked into a sample at specific stages throughout the qPCR process. Measuring the concentration of the spiked-in sequence allows for its use as a serial quality control and it can be used to monitor recovery efficiency and to identify false negative results such as the presence of PCR inhibitors in molecular detection workflows. This is of particular importance when working with sample types that have a high level of inhibition. For a 5X version of this control, please see Cat. No. A39176.
Note: this product contains a sequence for AmpC beta-lactamase and may amplify for any assays designed to detect this antibiotic resistance target.
研究用途にのみご使用ください。診断目的には使用できません。
仕様
フィデリティ(vs. Taq)5 X
フォーマットTube(s)
製品ラインTaqMan
製品タイプUniversal DNA Spike-In Control
数量1 mL
出荷条件Approved for shipment at Room Temperature or on Wet Ice
種Not Species-Specific
濃度5X
GC-Rich PCR Performance高
PCR法qPCR
Unit SizeEach
組成および保存条件
Store in freezer (-5°C to -30°C).
よくあるご質問(FAQ)
What is the size of TaqMan Universal DNA Spike-In Control (Cat. No. A39175)?
The TaqMan Universal DNA Spike-In Control (Cat. No. A39175) is a linearized plasmid less than 5 kb in size.
How can I check for the presence of inhibitors in my samples when running microbiota profiling experiments?
We offer a TaqMan Universal DNA Spike-In Control (Cat. No. A39175 or A39176) that can be added to samples before extraction, to monitor recovery efficiency.