PNGase F Glycan Cleavage Kit

製品番号（カタログ番号）	数量
A39245	1 kit

製品番号（カタログ番号） A39245

価格（JPY）

47,600

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1 kit

PNGアーゼF グリカン開裂キットには、糖タンパク質からほぼすべてのN結合型オリゴ糖を酵素で除去するために必要なすべての構成品が含まれています。このキットにはPNGアーゼF酵素が含まれており、高マンノース型、ハイブリッド型、および複合型オリゴ糖の最も内側のGlcNAc残基およびアスパラギン残基でN-グリカン鎖を切断します。

PNGアーゼFグリカン開裂キットの特長：
•≥純度95%
• エンドグリコシダーゼF1、F2、またはF3を含まない組換えPNGアーゼF酵素
• 酵素は、N-グリカンのコアオリゴ糖をインタクトなままにするため、下流分析に適しています
• ネイティブまたは変性条件下での使用に適しています

PNGアーゼF グリカン開裂キットに含まれる成分は以下のとおりです：PNGアーゼF 30,000単位（500,000単位/mL）およびPNGアーゼF10X緩衝液 1 mL。

研究用にのみ使用できます。診断用には使用いただけません。

形状Liquid

数量1 kit

出荷条件湿氷

製品タイプPNGase F Glycan Cleavage Kit

Unit SizeEach

組成および保存条件

• 組換えPNGアーゼF酵素30,000ユニット（500,000ユニット／mL）は、-20℃で保存してください
• PNGアーゼF10Xバッファー液1 mLは、-20℃で保存してください

よくあるご質問（FAQ）

In the PNGase Glycan Cleavage Kit, can I use PNGase F under native or denaturing conditions?

Yes, PNGase F enzyme may be used in both native and denaturing conditions

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

In the PNGase Glycan Cleavage Kit, how much PNGase F should I use to remove my carbohydrate under native or DTT denaturing conditions?

When the protein is not denatured with SDS, PNGase F has to work harder to reach the cleavage site of the carbohydrate (due to secondary and tertiary protein structures). In these instances, using more enzyme and/or extended incubation times may help. These conditions will need to be determined empirically for your specific protein.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

In the PNGase Glycan Cleavage Kit, is PNGase F compatible with downstream analysis such as HPLC and mass spectrometry?

PNGase F enzyme is stored in 50% glycerol. Therefore, HPLC and/or mass spectrometry is not compatible due to the fact that glycerol is not tolerated in instruments used for these applications.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

In the PNGase Glycan Cleavage Kit, what are the typical reaction conditions for PNGase F, Recombinant?

Typical reaction conditions are as follows:

Optimal incubation times and enzyme concentrations must be determined empirically for a particular substrate.
1. Combine 1-20 µg of glycoprotein, 1 µL of 10X denaturing buffer (5% SDS, 400 mM DTT) and H2O (if necessary) to make a 10 µL total reaction volume.
2. Denature glycoprotein by heating reaction at 100 degrees C for 10 min.
3. Make a total reaction volume of 20 µL by adding 2 µL PNGaseF 10X Buffer, 1-5 µL PNGaseF enzyme, 2 µL 10% NP-40 and H2O.
4. Incubate reaction at 37 degrees C for 1 hr.
Note: Reactions may be scaled-up linearly to accommodate larger reaction volumes.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

In the PNGase Glycan Cleavage Kit, what happens to the asparagine residue after PNGase F removes the sugar?

As PNGase F is a glycoamidase, the asparagine gets converted into aspartic acid.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.