What reagents and conditions are Zeba Desalting columns, plates, and cartridges compatible with?
Zeba Desalting columns, plates, and cartridges are compatible with a range of reagents and conditions. They have been confirmed to be compatible with the following:
- pH range of 3-10
- Mild oxidants/reductants
- Chaotropes (stable in 8 M urea and 6 M guanidine HCl)
- Salts
- Alcohols up to 20%
- Organic solvents such as dimethyl sulfoxide (DMSO) and dimethylformamide (DMF) (we recommend using a step-gradient of increasing concentration of the organic solvent during column equilibration)
Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.
With Zeba Spin Desalting columns, plates, and cartridges, how can I completely get rid of glycerol and detergent from my sample?
Glycerol and sugars add viscosity to a sample, while detergents create micelles, making the removal of each difficult. Although other methods are a better choice for removing these components, Zeba columns can be used by modifying the desalting protocol. Modifications include diluting the sample, processing a smaller volume of the sample, and adding the sample slowly to allow it to enter the gel and equilibrate into the pores before processing.
Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.
After desalting with your Zeba Spin Desalting columns, I still have leftover salt in my sample. What should I do to get rid of all the salt?
You can either reduce the volume of sample processed in each column (the product manual indicates the volume range recommended for each column size) or run the flow-through from one column over a fresh column.
Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.