Pierce™ MHC Class I Antibody Coupling Kit

The Thermo Scientific Pierce MHC Class I Antibody Coupling Kit is a comprehensive kitted solution designed for magnetic immunoprecipitation of MHC Class I protein and peptide complexes. It provides all necessary components for antibody coupling and peptide enrichment required for immunopeptidomic studies. This kit features an optimized protocol with guidance all the way through LC-MS analysis.

Utilizing Pierce Protein A/G Magnetic Agarose to deliver reproducible yields comparable to traditional agarose-based methods, when combined with Pierce C18 spin tips (Cat. No. 84850) and Pierce Peptide Retention Time Calibration Mixture (Cat. No. 88321), this kit enables complete isolation of MHC Class I-associated peptides (MAPs).

Features of the Pierce MHC Class I Antibody Coupling Kit include:

• Streamlined and standardized protocol—delivers reliable, consistent outcomes with accelerated processing times, enabling high-throughput analysis while maintaining data integrity and precision
• Reproducible rapid results—employs a refined, uniform methodology that ensures consistent procedural implementation, helping provide researchers with the confidence necessary for comparing results across multiple experiments and laboratory
• Automation compatible—designed for seamless integration with automated laboratory systems such as the KingFisher Apex system, facilitating scalable workflows and reducing manual intervention requirements

The immunoprecipitation workflow follows a straightforward three-step process: antibody coupling to the magnetic beads, mixing with the biological sample, and subsequent enrichment of MHC Class I protein and peptide complexes. This versatile approach accommodates a wide range of sample types, including cells, tissue specimens, and biofluids, providing researchers with flexibility in experimental design and sample selection for immunopeptidomic applications.

To further optimize downstream analysis and ensure maximum peptide recovery, the use of C18 tips is outlined in the provided protocol. This step is optimized to isolate the MAPs for improved mass spectrometry analysis and overall experimental outcomes. This standardization is particularly valuable in multi-site studies and collaborative research efforts where experimental consistency is paramount.