QuantStudio™ 1 Real-Time PCR System, 96-well, 0.2 mL, laptop

Citations & References (962)

Applied Biosystems™

QuantStudio™ 1 Real-Time PCR System, 96-well, 0.2 mL, laptop

Applied Biosystems QuantStudio 1 リアルタイム PCR システムは、品質と信頼性を実際の選択として提供します。この手頃な価格の装置は基本的な機能を提供し、qPCR詳細を見る

製品番号（カタログ番号）	数量
A40427	1 System

製品番号（カタログ番号） A40427

価格（JPY）

見積もりを依頼する

1 System

Applied Biosystems QuantStudio 1 リアルタイム PCR システムは、品質と信頼性を実際の選択として提供します。この手頃な価格の装置は基本的な機能を提供し、qPCR 初心者や予算の限られたお客様に理想的です。QuantStudio 1 システムは、その手軽さにも関わらず、品質と信頼性、そして弊社の QuantStudio 装置ファミリーにおける最新のユーザーエクスペリエンスを提供します。

Quantstudio 1 Design & Analysis Software > をダウンロードする | リリースノート

QuantStudio 1 システムの特長：
•タッチスクリーン—8.5 インチの大きなインタラクティブタッチスクリーン
•固定ブロック—1 つの固定 96 ウェル、開けやすい引き出し付きの 0.2 mL 等温ブロック
•反応容量—10–100μL
•マルチプレックス能力—もっとも一般的に使用される 3 種類の励起および蛍光フィルターを提供：FAM、VIC、および ROX
•プロトコル—事前最適化済みのプロトコルテンプレート
•ソフトウェア—ウェブブラウザまたはデスクトップコンピューターで解析をサポートする直観的なソフトウェア
• 色素—FAM/SYBR Green/SYTO 9、VIC/HEX/TET/JOE、および JUN/ROX/Texas Red 色素に対してキャリブレーションされた 3 種類のフィルター
• 感度—シングルプレックス反応において、わずか 1.5 倍量の違いを検出
• 寸法（H x W x D）および重量—40 x 27 x 50 cm、<26 kg

Thermo Fisher Connect
クラウドサービスによる Thermo Fisher Connect は、データの保管、解析、そして共有のための安全な場所を科学者に提供します。機器からデータをアップロードしたり、ウェブアクセス可能なあらゆる場所からご自身のファイルをアップロードしたりすることが可能です。Thermo Fisher Connect 内の Applied Biosystems qPCR 解析モジュールは、フレキシブルで高速、そして使いやすいウェブブラウザベースのソフトウェアです。

使いやすさ
QuantStudio 1 リアルタイム PCR システムは、コンパクトな形状、出荷前キャリブレーション済み、およびユーザーフレンドリーな包装で提供されるため、迅速かつシンプルな設置で即時使用が可能です。インタラクティブタッチスクリーンが現代的な利便性を提供し、シンプル化された装置ソフトウェアおよび事前最適化済みのプロトコルテンプレートが、素早いセットアップと分析開始のためのあらゆる経験レベルを可能にします。

デザインおよび解析ソフトウェア

オプション 1：QuantStudio デザインおよび解析クラウドソフトウェア
• PC または MAC で操作できるウェブブラウザベースシステムをサポート
• 利便性と反応時間の向上のための合理的なソフトウェア
• Thermo Fisher Connect を介して、いつでもどこでも安全なアクセスが可能
• ソフトウェアのインストール、ライセンス、バージョンアップデートが不要

オプション 2：QuantStudio デザインおよび解析デスクトップソフトウェア
• Windows™ 7 および Windows™ 10 OS における従来同様のコンピューターシステム構成をサポート
• 利便性と反応時間の向上のための合理的なソフトウェア
• ライセンスの購入が不要

信頼性の高い結果
ウェル間および装置間のデータ正確性が、OptiFlex テクノロジーによって改善されており、これは白色 LED と 3 種類の励起および蛍光フィルターチャンネルを特長とします。

感度の高いデータ解析
シングルプレックス反応において、わずか 1.5 倍量のターゲットの変化を検出でき、 10 logs のリニアダイナミックレンジが得られます。

高いアプリケーション汎用性
遺伝子発現、遺伝的変異、遺伝子制御などを含む幅広いアプリケーションにおいて、シンプルで最適化されたプロトコル、信頼できるアッセイと試薬、および直観的なソフトウェアが利用できます。

研究用途にのみご使用ください。診断目的には使用できません。

奥行き（メートル法）50 cm

ディスプレイタイプ8.5 in. Touchscreen

使用対象 (装置)QuantStudio™

フォーマット96-well Plate

高さ（メートル法）40 cm

製品ラインApplied Biosystems™ QuantStudio

数量1 System

反応量範囲10 to 100 μL

出荷条件Approved for Shipment at Room Temperature or on Wet Ice

重量<26 kg

幅（メートル法）27 cm

ブロック構成96-well

ブロックフォーマットNon-interchangeable

Unit SizeEach

組成および保存条件

Laptop

よくあるご質問（FAQ）

What is the difference between continuous melt curves and step and hold melt curves on the Applied Biosystems Real-Time PCR Instruments?

Continuous melt curve: increases the temperature by the ramp increment (degrees C/sec) so that the temperature will change at a constant rate set in the protocol.
Step and Hold melt curve: increases the temperature by the ramp increment (degrees C) and then holds at that temperature for the time specified by the user.

The amount of data collected will depend on the filter(s) selected. The more filters you use, the longer the acquisition will take and the fewer data points will be collected.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Can I import a standard curve for my real-time PCR data into the Design & Analysis Software (v2.x)?

It is possible to import a standard curve into the Design & Analysis Software (v2.x) if you are analyzing results from one of the following Applied Biosystems real-time PCR instruments:
• 7900HT Fast Real-Time PCR System
• 7500/7500 Fast Real-Time PCR System
• StepOne/StepOne Plus Real-Time PCR System
• ViiA 7 Real Time PCR System
• QuantStudio 1 Real-Time PCR System
• QuantStudio 3 & 5 Real-Time PCR System
• QuantStudio 6 & 7 Flex Real-Time PCR System
• QuantStudio 6 & 7 Pro Real-Time PCR Systems
• QuantStudio 12K Flex Real-Time PCR system

Follow the instructions below to import a standard curve for analysis in the Design & Analysis Software (v2.x):
1. Open the data file in the Design & Analysis Software (v2.x).
2. Click on Actions.
3. Click on "Standard Curve Analysis Setting".
4. Select "External Standard Curves" and click on "Import".
5. Browse to the proper .csv file and click "Open".
6. Click "Apply".

Can I import a standard curve for my real-time PCR data into the QuantStudio Design & Analysis Software (v1.x)?

If you are analyzing results from the QuantStudio 1 Real-Time PCR System, QuantStudio 3 Real-Time PCR System, or QuantStudio 5 Real-Time PCR System, it is possible to import a standard curve into the QuantStudio Design & Analysis Software (v1.x) following the instructions below:
1. Open the data file in the QuantStudio Design & Analysis Software (v1.x).
2. Go to Analysis Settings.
3. Click on the "Standard Curve Settings" tab.
4. Select the option to "Use a standard curve imported from another experiment" and click on "Import".
5. Browse to and highlight the proper data file and click "Select".
6. Click Apply.

With the QuantStudio 1 instrument, why am I only able to export my run files to USB, whereas the cloud and network options are not available?

It is possible that the instrument is not connected to the computer and is not linked with the Thermo Fisher Cloud. Please see page 38 of the QuantStudio 1 Real-Time PCR System User Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017853_QS1%20UG.pdf) for instructions to link an instrument profile with the Thermo Fisher Cloud. Although default installation instructions recommend using an Ethernet cable, it can also be done wirelessly using a High Power USB WiFi Module, Cat. No. A26774 (https://www.thermofisher.com/order/catalog/product/A26774).

If you experience connection issues between the instrument and computer, it might be useful setting a static IP address on both the instrument and computer. On the computer, you can use online help, such as this one (https://kb.netgear.com/27476/How-do-I-set-a-static-IP-address-in-Windows) to set the static IP address. Here are the details for the settings:

- Computer IP: 10.0.0.2
- Instrument IP: 10.0.0.3
- Subnet: 255.255.255.0
- DNS1: 8.8.8.8
- DNS2: 8.8.4.4
- Default gateway: 10.0.0.1

On the instrument, the IP address can be found under Settings and About Instrument. If you see an IP address but are still having network issues, try to PING the IP Address in a command window of the computer:

- Select the windows start button and select the RUN command.
- Open the Run command and type in CMD.
- This will open the Command window.
- Type the word "PING", space, followed by the IP address and press enter.
- You should see 4 packets of data sent, 4 received, and 0 lost.

If you PING the IP Address successfully (4 sent, 4 received, 0 lost), but still cannot connect, it means that one of the needed ports is still not open. Please contact your IT department and review the instrument checklist.
If you PING the IP address unsuccessfully, please send us the serial number of the instrument so that we can contact your local engineer.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Where can I find the software and newest firmware for the QuantStudio 1 instrument?

In an effort to reduce environmental impact, we no longer supply the software on the CD. Please download it from here (https://www.thermofisher.com/uk/en/home/global/forms/life-science/quantstudio-software-download.html).

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

View all QuantStudio™ 1 Real-Time PCR System, 96-well, 0.2 mL, laptop FAQs

引用および参考文献 (962)

引用および参考文献

Abstract

Comparable type I interferon score determination from PAXgene and Tempus whole blood RNA collection and isolation systems.

Authors:Lamot L,Niemietz I,Brown KL

Journal:BMC research notes

PubMed ID:31416482

OBJECTIVE: Type I interferons (IFN) have important roles in many immune-mediated inflammatory diseases (IMIDs) and are a relatively new therapeutic target. Direct detection of type I IFNs has proved challenging, thus their presence is often inferred from the expression of interferon-stimulated genes (ISGs) and calculation of an interferon score (IS). ... More

Efficient gene editing of human long-term hematopoietic stem cells validated by clonal tracking.

Authors:Ferrari S,Jacob A,Beretta S,Unali G,Albano L,Vavassori V,Cittaro D,Lazarevic D,Brombin C,Cugnata F,Kajaste-Rudnitski A,Merelli I,Genovese P,Naldini L

Journal:Nature biotechnology

PubMed ID:32601433

Targeted gene editing in hematopoietic stem cells (HSCs) is a promising treatment for several diseases. However, the limited efficiency of homology-directed repair (HDR) in HSCs and the unknown impact of the procedure on clonal composition and dynamics upon transplantation have hampered clinical translation. Here, we apply a barcoding strategy to ... More

Tamoxifen induction of Cre recombinase does not cause long-lasting or sexually divergent responses in the CNS epigenome or transcriptome: implications for the design of aging studies.

Authors:Chucair-Elliott AJ,Ocanas SR,Stanford DR,Hadad N,Wronowski B,Otalora L,Stout MB,Freeman WM

Journal:GeroScience

PubMed ID:31493147

The systemic delivery of tamoxifen (Tam) to activate inducible CreERT2-loxP transgenic mouse systems is now widely used in neuroscience studies. This critical technological advancement allows temporal control of DNA-cre recombination, avoidance of embryonically lethal phenotypes, and minimization of residual cell labeling encountered in constitutively active drivers. Despite its advantages, the ... More

CD4(+) CCR6(+) T cells, but not γδ T cells, are important for the IL-23R-dependent progression of antigen-induced inflammatory arthritis in mice.

Authors:Razawy W,Asmawidjaja PS,Mus AM,Salioska N,Davelaar N,Kops N,Oukka M,Alves CH,Lubberts E

Journal:European journal of immunology

PubMed ID:31778214

ILâ23 plays an important role in the development of arthritis and the ILâ23 receptor (ILâ23R) is expressed on different types of TÂ cells. However, it is not fully clear which ILâ23R(+) TÂ cells are critical in driving TÂ cellâmediated synovitis. We demonstrate, using knockâin ILâ23RâGFP reporter (ILâ23R(GFP/+)) mice, that CD4(+)CCR6(+) TÂ cells and Î³Î´ ... More

Inactivation of Pepper Mild Mottle Virus in Water by Cold Atmospheric Plasma.

Authors:Filipić A,Dobnik D,Tušek Žnidarič M,Žegura B,Štern A,Primc G,Mozetič M,Ravnikar M,Žel J,Gutierrez Aguirre I

Journal:Frontiers in microbiology

PubMed ID:33584622

Water scarcity is one of the greatest threats for human survival and quality of life, and this is increasingly contributing to the risk of human, animal and plant infections due to waterborne viruses. Viruses are transmitted through polluted water, where they can survive and cause infections even at low concentrations. ... More

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