CTS™ Opti-MEM™ I Medium
CTS™ Opti-MEM™ I Medium
Gibco™

CTS™ Opti-MEM™ I Medium

Gibco CTS Opti-MEM I Medium is an improved minimal essential medium (MEM) that is recommended for use with the CTS詳細を見る
テクニカルサポートオーダーサポート
表示を変更buttonViewtableView
製品番号（カタログ番号）数量
A4124801100 mL
A4124802500 mL
製品番号（カタログ番号） A4124801
価格（JPY）
3,800
Each
お問い合わせください ›
数量:
100 mL

Gibco CTS Opti-MEM I Medium is an improved minimal essential medium (MEM) that is recommended for use with the CTS LV-MAX Transfection Kit as a complexation buffer. This medium contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. CTS Opti-MEM I uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH. The formulation of CTS Opti-MEM I is identical to research-grade Opti-MEM I Reduced Serum Medium.

CTS Opti-MEM I is xeno-free and contains human transferrin.

CTS Opti-MEM I is manufactured in conformity with cGMP for medical devices, 21 CFR, Part 820, and certified to the ISO 13485. Drug Master File and Regulatory Support File available upon request.

Custom configuration and packaging available upon request.

細胞ベース、遺伝子ベース、または組織ベースの製品の研究での用途または製造。注意：ヒトまたは動物への直接投与は意図されていません。
仕様
細胞株Viral Production Cells
細胞タイプHEK293
使用対象（アプリケーション）LV Production
フォーマットBottle
製造品質ISO 13485, MDSAP, FDA-registered, 21 CFR 820
製品タイプMinimum Essential Medium (MEM)
数量100 mL
品質保持期間18 months from date of manufacture
出荷条件Ambient
分類Animal Origin-free
形状Liquid
無菌性Sterile-filtered
Unit SizeEach
組成および保存条件
100 mL bottle. Store at 2°C to 8°C and protect from light.

よくあるご質問（FAQ）

Why do you recommend using V bottom 96-well plates for harvesting lentivirus from CTS Viral Production Cells?

We recommend using V-bottom 96-well plates to harvest the supernatant containing lentiviral particles as they allow for easier detection of the cell pellets after centrifugation of the cultures.

During lentivirus production from CTS Viral Production Cells, will the shaker vibration affect the cell growth of other cultures maintained in the same incubator or in stacked incubators?

We recommend maintaining shaking cultures in a separate incubator from all other incubators to avoid vibration effects. Alternatively, we recommend using a small, quiet shaker such as the Thermo Scientific CO2 Resistant Shaker (Cat. No. 88881102) for convenience and best performance.

Is 8% CO2 a growth requirement for CTS Viral Production Cells?

We generally recommend culturing the CTS Viral Production Cells under 8% CO2 conditions. We found that cells cultured in 5% CO2 grew slower and were more prone to more cell clumping than when grown in 8% CO2.

What is special about the CTS LV-MAX lentivirus production system?

The CTS LV-MAX lentivirus production system is a fully-optimized complete system in which the cells, media, and transfection reagent work together to generate high-titer lentivirus. The CTS LV-MAX Production Medium is specifically designed to work with the system, to support high density suspension cell growth of CTS Viral Production Cells.

The manual recommends using CTS Viral Production Cells passaged to P5 (˜3 weeks) on the day of transfection. Are these general cell culture guidelines or special requirements for these cells?

Although 3-4 passages before transfection are sufficient for most cell lines recovering directly from liquid N2 storage, we found that CTS Virus Production Cells passaged to P5 showed the highest performance in lentivirus particle production and reproducibility between batches. Therefore, we recommend passaging the cells to P4 at the end of a given week for lentivirus production the following week.

View all CTS™ Opti-MEM™ I Medium, 100 mL FAQs