CTS™ LV-MAX™ Transfection Kit
CTS™ LV-MAX™ Transfection Kit
Gibco™

CTS™ LV-MAX™ Transfection Kit

The Gibco CTS LV-MAX Transfection Kit is an essential component of the CTS LV-MAX lentiviral production system. Based on extensive詳細を見る
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製品番号（カタログ番号）数量
A41326011 Kit (For 1 L of LV Production)
A41326021 Kit (For 10 L of LV Production)
製品番号（カタログ番号） A4132601
価格（JPY）
243,200
Each
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数量:
1 Kit (For 1 L of LV Production)

The Gibco CTS LV-MAX Transfection Kit is an essential component of the CTS LV-MAX lentiviral production system. Based on extensive Design of Experiment (DOE) methodologies, the cationic lipid-based transfection reagent, lentiviral production supplement, and LV enhancer are specifically screened for maximal delivery of multiple DNA plasmids to achieve the highest possible lentiviral titer when used in conjunction with CTS Viral Production Cells cultured in CTS LV-MAX Production Medium.

All CTS LV-MAX Transfection Kit components are produced under cGMP manufacturing conditions with extensive quality-control testing to ensure proper preclinical, clinical, and commercial therapeutic viral vector applications.

CTS LV-MAX Transfection Kit features include:

  • Uniquely designed to enhance transient transfection of multiple DNA plasmids into high density suspension HEK293-derived cell culture
  • Enables high viral titers of up to 1 x 108 TU/mL (unconcentrated LVV-GFP) to allow for a cost-effective alternative to PEI-based transfection reagents
  • Scalable protocol for 96-deep well blocks, flasks, and bioreactors
  • Regulatory-compliant reagents produced in conformity with cGMP for medical devices, 21 CFR, Part 820, and certified to the ISO 13485. Drug Master File and Regulatory Support File available upon request.

The CTS LV-MAX Transfection Kit requires CTS Opti-MEM I (not included in this kit) for complexation. The chemically-defined, serum-free, animal origin-free features of the CTS LV-MAX Transfection Kit result in high reproducibility and lot-to-lot reliability. The formulation of this product is identical to the research-grade LV-MAX Transfection Kit, but the CTS LV-MAX Transfection Kit is validated for clinical and commercial therapeutic applications.

Custom configuration and packaging available upon request.

細胞ベース、遺伝子ベース、または組織ベースの製品の研究での用途または製造。注意：ヒトまたは動物への直接投与は意図されていません。
仕様
分類動物由来成分不含
培養タイプMammalian
使用対象（アプリケーション）LV Production
製造品質ISO 13485, FDA-registered, 21 CFR 820
製品タイプトランスフェクションキット
数量1 Kit (For 1 L of LV Production)
試薬タイプCationic Lipid-Based Transfection
品質保持期間12 months from date of manufacture
出荷条件Gel Ice
細胞タイプHEK293
フォーマットBottle
Unit SizeEach
組成および保存条件
• CTS LV-MAX Supplement, 50 mL
• CTS LV-MAX Transfection Reagent, 6 mL
• CTS LV-MAX Enhancer, 40 mL

Store at 2–8°C and protect from light. Ships on gel ice.

よくあるご質問（FAQ）

Why do you recommend using V bottom 96-well plates for harvesting lentivirus from CTS Viral Production Cells?

We recommend using V-bottom 96-well plates to harvest the supernatant containing lentiviral particles as they allow for easier detection of the cell pellets after centrifugation of the cultures.

During lentivirus production from CTS Viral Production Cells, will the shaker vibration affect the cell growth of other cultures maintained in the same incubator or in stacked incubators?

We recommend maintaining shaking cultures in a separate incubator from all other incubators to avoid vibration effects. Alternatively, we recommend using a small, quiet shaker such as the Thermo Scientific CO2 Resistant Shaker (Cat. No. 88881102) for convenience and best performance.

Is 8% CO2 a growth requirement for CTS Viral Production Cells?

We generally recommend culturing the CTS Viral Production Cells under 8% CO2 conditions. We found that cells cultured in 5% CO2 grew slower and were more prone to more cell clumping than when grown in 8% CO2.

What is special about the CTS LV-MAX lentivirus production system?

The CTS LV-MAX lentivirus production system is a fully-optimized complete system in which the cells, media, and transfection reagent work together to generate high-titer lentivirus. The CTS LV-MAX Production Medium is specifically designed to work with the system, to support high density suspension cell growth of CTS Viral Production Cells.

The manual recommends using CTS Viral Production Cells passaged to P5 (˜3 weeks) on the day of transfection. Are these general cell culture guidelines or special requirements for these cells?

Although 3-4 passages before transfection are sufficient for most cell lines recovering directly from liquid N2 storage, we found that CTS Virus Production Cells passaged to P5 showed the highest performance in lentivirus particle production and reproducibility between batches. Therefore, we recommend passaging the cells to P4 at the end of a given week for lentivirus production the following week.

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