AAV-MAX Transfection Kit
AAV-MAX Transfection Kit
Gibco™

AAV-MAX Transfection Kit

The AAV-MAX Transfection Kit is an essential component of the AAV-MAX Helper-Free AAV Production System. It is formulated for the transient transfection of Viral Production Cells 2.0.
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製品番号（カタログ番号）数量
A505161 kit for 10 L of production
A505151 kit for 1 L of production
製品番号（カタログ番号） A50516
価格（JPY）
1,193,000
Each
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数量:
1 kit for 10 L of production

The Gibco AAV-MAX Transfection Kit is an essential component of the AAV-MAX Helper-Free AAV Production System. It is formulated for the transient transfection of Viral Production Cells 2.0.

The kit includes a cationic lipid-based transfection reagent, as well as a transfection booster and production enhancer, which are designed to work together to produce adeno-associated virus (AAV) particles from Viral Production Cells 2.0 when cultured in Viral Production Medium.

Features of the AAV-MAX Transfection Kit include:

  • Components work synergistically within the AAV-MAX system to produce high AAV titers greater than 5 x 1010 vg/mL (unpurified)
  • AAV-MAX transfection reagent—cationic lipid-based transfection reagent for helper virus-free triple transfection of high-density cultures
  • AAV-MAX booster—chemically defined component that works with the transfection reagent to achieve high transfection efficiency
  • AAV-MAX enhancer—chemically defined component that enhances the production of AAV particles
  • Robust transfection protocols are adaptable to large-scale transfection
研究用途にのみご使用ください。診断目的には使用できません。
仕様
細胞株HEK293
分類Animal Origin-free
培養タイプ懸濁細胞培養
概要Serum-free, animal origin-free, chemically defined
発現システムMammalian
使用対象（アプリケーション）AAV Production
使用対象 (装置)AAV-MAX System
内容100 mL Gibco AAV-MAX Enhancer, 60 mL Gibco AAV-MAX Transfection Reagent, 30 mL Gibco AAV-MAX Supplement
製造品質ISO 13485, ISO 9001 certified
パッケージングタイプBottle
製品ラインAAV-MAX
製品タイプAAV production transfection Kit
数量1 kit for 10 L of production
試薬タイプTransfection kit
品質保持期間12 months from date of manufacture
出荷条件Wet Ice
無菌性Sterile-filtered
細胞タイプEstablished Cell Lines
フォーマットKit
サンプルタイプPlasmid DNA
Transfection TechniqueLipid-based Transfection, Transient Transfection
Unit SizeEach
組成および保存条件
• 60 mL AAV-MAX Trasfection Reagent
• 30 mL AAV-MAX Transfection Booster
• 100 mL AAV-MAX Enhancer

Reagents should be stored at 2–8°C. Do not freeze.

よくあるご質問（FAQ）

In the AAV-MAX Transfection Kit (Cat. No. A50515, A50516), what is the AAV-MAX Enhancer and what does it do?

The AAV-MAX Enhancer increases AAV vector production in Viral Production Cells (VPCs) 2.0. The identity and mechanism of action of the AAV-MAX Enhancer are proprietary.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

In the AAV-MAX Transfection Kit (Cat. No. A50515, A50516), what is the AAV-MAX Transfection Booster and what does it do?

The AAV-MAX Transfection Booster enhances the efficiency of co-transfection of multiple plasmids into high-density Viral Production Cells (VPCs) 2.0. The identity and mechanism of action of the transfection booster are proprietary.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I use other transfection reagents, such as PEI (polyethylenimine), in the AAV-MAX Helper-Free AAV Production System?

The AAV-MAX Transfection Reagent, AAV-MAX Tranfection Booster, and AAV-MAX Enhancer are specifically designed to work synergistically with the Viral Production Medium and Viral Production Cells 2.0 to provide maximum titers. Mixing and matching of reagents is not recommended, as this will decrease the overall performance.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

How scalable is the AAV-MAX transfection protocol?

The AAV-MAX transfection protocol is scalable from 24 deep-well plates to shake flasks to bioreactors. The user guide provides guidance on this.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Are the LV-MAX Enhancer and AAV-MAX Enhancer different?

Yes, these are different, and each is optimized for its intended use.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

View all AAV-MAX Transfection Kit FAQs