Viral Production Cells 2.0 are a clonal cell line derived from the HEK293F parental cell line and a core component of the AAV-MAX Helper-Free AAV Production System.
Gibco Viral Production Cells 2.0 are a clonal cell line derived from the HEK293F parental cell line and a core component of the AAV-MAX Helper-Free AAV Production System.
Adapted to the chemically defined Viral Production Medium and maintained in suspension culture in that medium
Highly transfectable using the AAV-MAX Transfection Kit for adeno-associated virus (AAV) production
Supplied frozen in a vial containing 1 mL of cells at 1 x 107 viable cells/mL in Viral Production Medium and 10% DMSO
Should be thawed directly into Viral Production Medium supplemented with 4 mM GlutaMAX Supplement (Cat. No. 35050038) for optimal performance
Clonal, 293F-derived, high-producer cell line
Absence of SV40 large T antigen
Optimized for high-density, suspension culture (>12 million cells/mL) in chemically defined medium
Enable production of high AAV titers, greater than 5 x 1010 vg/mL (unpurified)
Robust scalability and passage stability
研究用途にのみご使用ください。診断目的には使用できません。
仕様
セルの状態Frozen
濃度1e7 cells/mL
使用対象(アプリケーション)AAV Production
フォーマット6 Vials
推奨培地Gibco Viral Production Medium
セル数1 x 107 cells
製品ラインAAV-MAX
製品タイプViral Production Cells
数量6 x 1 mL
出荷条件dry ice for domestic shipment; liquid nitrogen for international shipment
電子線源Human
細胞株Viral Production Cells 2.0
細胞タイプ腎臓(胚性)
Culture Type懸濁細胞培養
形状Frozen
種ヒト
Unit SizeEach
組成および保存条件
6 cryovials, store in vapor phase liquid nitrogen
よくあるご質問(FAQ)
Can Viral Production Cell 2.0 (Cat. No. A49784, A51218) only grow in suspension, or can they be adherent using specific medium conditions?
Viral Production Cells 2.0 were designed specifically for suspension cultures. We did not validate their usage with adherent culture and therefore do not recommend doing so.
Can I use Viral Production Cells (Cat. No. A35347, A35827) with the AAV MAX Helper-Free AAV Production System?
The Viral Production Cells 2.0 are specifically designed to work with the other components of the AAV MAX Helper-Free AAV Production System to provide maximal adeno-associated virus (AAV) titers. Viral Production Cells could be used but we don't recommend doing this as it will reduce system performance.
What is the highest density that Viral Production Cells 2.0 can be cultured at?
The Viral Production Cells 2.0 achieve maximum cell densities of >1.2 x 10E7 cells/mL in shake flask cultures.
What is the passage stability of the Viral Production Cells 2.0?
We recommend using the Viral Production Cells 2.0 between passages 3 to 25 for adeno-associated virus (AAV) production.
How long do the Viral Production Cells 2.0 require to recover post-thaw before transfection?
We recommend allowing freshly thawed Viral Production Cells 2.0 to recover in culture for at least three passages prior to transfection.