Fetal Bovine Serum, value (formerly USDA-approved in North America or qualified, Brazil in other regions)

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Gibco™

Fetal Bovine Serum, value (formerly USDA-approved in North America or qualified, Brazil in other regions)

Name: Fetal Bovine Serum, value (formerly USDA-approved in North America or qualified, Brazil in other regions)
SKU: A5256701

Gibcoウシ胎児血清は基礎的な細胞培養、特殊研究、および特定のアッセイなどに優れた価値をもたらし、研究ニーズや予算要件を満たすのに役立つ一貫した品質と受賞歴のあるサポートにより、研究者の信頼を得ています。血清カテゴリー：バリュー • 丈夫な細胞株を使用する細胞培養用の血清 • 基礎研究に優れた価値を提供詳細を見る

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表示を変更

製品番号（カタログ番号）	数量	フォーマット	熱失活	Gamma-irradiated
A5256701	500 mL	ボトル	No	No
A5209401	50 mL	ボトル	No	No
A5209402	10 x 50 mL	ボトル	No	No
A5209501	50 mL	One Shot	Yes	No
A5209502	10 x 50 mL	One Shot	Yes	No
A5256501	500 mL	Bottle	No	Yes
A5256801	500 mL	Bottle	Yes	No
A5256901	500 mL	Bottle	Yes	Yes

8 オプション

製品番号（カタログ番号） A5256701

価格（JPY）

お問い合わせください ›

数量:

500 mL

フォーマット:

ボトル

熱失活:

Gamma-irradiated:

Gibcoウシ胎児血清は基礎的な細胞培養、特殊研究、および特定のアッセイなどに優れた価値をもたらし、研究ニーズや予算要件を満たすのに役立つ一貫した品質と受賞歴のあるサポートにより、研究者の信頼を得ています。

血清カテゴリー：バリュー
• 丈夫な細胞株を使用する細胞培養用の血清
• 基礎研究に優れた価値を提供
• エンドトキシン濃度：≤ 20 EU/mL（濃度は通常≤ 10 EU/mL）
• ヘモグロビン濃度：≤ 25 mg/dL
• 原産国：特定の原産国についてはCoAを参照してください

• 輸入規制により、すべての製品がすべての地域でご利用になれるとは限りません

• 米国への輸入が米国農務省（USDA）により承認されている地域でブルータングおよびアカバネが存在しないことがUSDA安全性試験で検査済みです

Gibco血清は以下を提供します：
• ISO 13485認証取得済み、FDA登録施設で加工
• 0.1 µmで3回ろ過
• Gibcoボトルはクリーンベンチなどのフード内での使用が容易で、汚染のリスクを低減し、細胞培養をより一貫して行うのに役立ちます
• ラベルの剥離式ステッカーにはロット番号と有効期限が記載されており、容易に参照できます

For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.

仕様

概要Gibco A5256701

エンドトキシン濃度≤20 EU/mL

使用対象（アプリケーション）*FBS import requirements vary globally, and acceptance depends on whether competent authorities allow import from a specific country of origin. Please check applicable regulations for the export of FBS and any FBS-containing products (e.g., cultured cells). Contact your Thermo Fisher Scientific representative for support.

Gamma-irradiatedNo

グリーン機能廃棄物の低減

熱失活No

ヘモグロビン濃度≤25 mg/dL

処理Triple Filtered at 0.1 μm

純度または品質グレードValue

品質保持期間5年間

出荷条件ドライアイス

種畜牛/ウシ

試験済み9CFRウイルス試験*、エンドトキシン試験、性能試験を含む最大50項目の品質試験(*注記:英国で製造されている場合、EMAにより検査される狂犬病およびブルータングウイルスを除く完全な9CFR)

USDA食品安全性検査を含むUSDA Safety Testing Performed for Mexican and Australian origins

Age胎児

原産国特定の原産国についてはCoAを参照してください*

形状Liquid

フォーマットボトル

製品タイプウシ胎仔血清

数量500 mL

Serum TreatmentStandard (Sterile-filtered)

無菌性滅菌済み

Sterilization Method3回ろ過済み、0.1 μm

Unit SizeEach

組成および保存条件

–10°C以下で保存。

よくあるご質問（FAQ）

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol¹.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How much of the total protein measured in Fetal Bovine Serum (heat inactivated or otherwise) is attributed to albumin?

Our CoAs only capture the total protein content not specifically the amount attributed to albumin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How much Fetal Bovine Serum (FBS) do I need to add to my medium?

FBS is added to culture medium at a concentration of 2-10% to provide attachment factors, nutrients, and hormones for mammalian cells, as well as to be a buffer against disruptions like pH changes and endotoxins. FBS has significant amounts of embryonic growth promoting factors like hormones, carrier proteins, and macromolecular proteins. It also has low levels of antibodies and other growth-inhibiting components. For most basal medium, 10% FBS is used. Some applications require up to 20% supplementation, so researchers need to determine what is optimal for their specific application and cell line.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I thaw Gibco Fetal Bovine Serum?

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Fetal Bovine Serum, value (formerly USDA-approved in North America or qualified, Brazil in other regions) FAQs

引用および参考文献 (1)

引用および参考文献

Abstract

Non-viral-mediated gene transfer of OX40 ligand for tumor immunotherapy.

Authors:Rakitina OA,Kuzmich AI,Bezborodova OA,Kondratieva SA,Pleshkan VV,Zinovyeva MV,Didych DA,Sass AV,Snezhkov EV,Kostina MB,Koksharov MO,Alekseenko IV

Journal:Frontiers in immunology

PubMed ID:39007148

BACKGROUND: Immune checkpoint blockade (ICB) is rapidly becoming a standard of care in the treatment of many cancer types. However, the subset of patients who respond to this type of therapy is limited. Another way to promote antitumoral immunity is the use of immunostimulatory molecules, such as cytokines or T ... More