Fetal Bovine Serum, Premium

Gibco™

Fetal Bovine Serum, Premium

Name: Fetal Bovine Serum, Premium
SKU: A5670701

Gibco fetal bovine sera offer an excellent value for basic cell culture, specialty research, and specific assays.

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製品番号（カタログ番号）	数量	フォーマット	熱失活	Gamma-irradiated
A5670701	500 mL	Bottle	No	No
A5670201	500 mL	Bottle	Yes	Yes
A5670401	50 mL	One Shot	No	No
A5670402	10 x 50 mL	One Shot	No	No
A5670501	50 mL	One Shot	Yes	No
A5670502	10 x 50 mL	One Shot	Yes	No
A5670801	500 mL	Bottle	Yes	No
A5670901	500 mL	Bottle	No	Yes

8 オプション

製品番号（カタログ番号） A5670701

価格（JPY）

お問い合わせください ›

数量:

500 mL

フォーマット:

Bottle

熱失活:

Gamma-irradiated:

Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements.

Sera category: Premium

Use for general cell culture with common cell lines
Low endotoxin and hemoglobin levels
Endotoxin level: <10 EU/mL
Hemoglobin level: <25 mg/dL
Origin: refer to CoA for specific origin. All products may not be available in all regions due to importation regulations.
US Department of Agriculture (USDA) safety tested for the absence of bluetongue and akabane from countries that are USDA-approved for importation into the US

Gibco serum delivers

ISO 13485–certified; processed in FDA registered facilities
Triple-filtered at 0.1 μm
Gibco bottle easier to use in hood; helps reduce risk of contamination and improve consistency
Peel-off sticker on label provides handy reference for lot number and expiration date recording in lab notebook

* FBS import requirements vary globally, and acceptance depends on whether competent authorities allow import from a specific country of origin. Please check applicable regulations for the export of FBS and any FBS-containing products (e.g., cultured cells). Contact your Thermo Fisher Scientific representative for support.

For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.

仕様

エンドトキシン濃度≤10 EU/mL

使用対象（アプリケーション）*FBS import requirements vary globally, and acceptance depends on whether competent authorities allow import from a specific country of origin. Please check applicable regulations for the export of FBS and any FBS-containing products (e.g., cultured cells). Contact your Thermo Fisher Scientific representative for support.

Gamma-irradiatedNo

グレードPremium

グリーン機能Less waste

熱失活No

ヘモグロビン濃度≤25 mg/dL

純度または品質グレードPremium

品質保持期間5 Years

出荷条件Dry Ice

種Cattle/Bovine

試験済みUp to 60 quality tests including 9CFR virus testing, endotoxin, and performance

Age胎児

原産国特定の原産国についてはCoAを参照してください*

形状凍結

フォーマットBottle

製品タイプFetal Bovine Serum

数量500 mL

無菌性滅菌ろ過済み

Sterilization MethodTriple-filtered, 0.1 μm

Unit SizeEach

組成および保存条件

Store at ≤-10°C.

よくあるご質問（FAQ）

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol¹.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How much of the total protein measured in Fetal Bovine Serum (heat inactivated or otherwise) is attributed to albumin?

Our CoAs only capture the total protein content not specifically the amount attributed to albumin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How much Fetal Bovine Serum (FBS) do I need to add to my medium?

FBS is added to culture medium at a concentration of 2-10% to provide attachment factors, nutrients, and hormones for mammalian cells, as well as to be a buffer against disruptions like pH changes and endotoxins. FBS has significant amounts of embryonic growth promoting factors like hormones, carrier proteins, and macromolecular proteins. It also has low levels of antibodies and other growth-inhibiting components. For most basal medium, 10% FBS is used. Some applications require up to 20% supplementation, so researchers need to determine what is optimal for their specific application and cell line.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I thaw Gibco Fetal Bovine Serum?

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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