MegaScript™ T7 Transcription Kit Plus

The MegaScript T7 Transcription Kit Plus contains all the reagents and buffers necessary for in vitro synthesis of large amounts of RNA. Each 20-μL reaction yields an average of 100 μg of RNA, while the scaled-up protocol yields up to 1 mg of RNA. This kit is suitable for full- or partially-modified-nucleotide substitution to produce modified RNA. It is also suitable for co-transcriptional capping with cap analogs to make capped RNA that can be translated into protein. Easily scale from RUO to GMP with the incorporation of TheraPure reagents, eliminating reoptimization during scale up.

Features of the MegaScript T7 Transcription Kit Plus include:

• Optimized to achieve high mRNA yields (>5 mg/mL)
• Flexible—allows use of modified nucleotides and/or cap analogs such as ARCA or CleanCap
• Includes GFP control template
• Made up of high quality reagents (TheraPure); eliminates the need to qualify new reagents during scale-up
• Available in two sizes: 50 and 1000 reactions

The MegaScript T7 Transcription Kit Plus requires a linearized DNA template with a T7 RNA polymerase promoter to create >5 mg/mL of RNA. MEGAscript kits are intended for the synthesis of large amounts of unlabeled or low-specific-activity RNA for a variety of uses, including transfections, preclinical mRNA therapeutic mRNA studies, microinjections, in vitro translation, and RNA structure and function analysis. Note that this kit does not create capped RNA, which is necessary for translational studies. MEGAscript kits are not recommended for synthesis of high-specific-activity probes.