Quantifiler™ Trio Automated DNA Quantification Kit

The Applied Biosystems Quantifiler Trio Automated DNA Quantification Kit is a robust solution that empowers forensic laboratories with accurate and efficient DNA analysis. Included is the Quantifiler Automation Enhancer for use with automated liquid handlers to prevent bubbles during pipetting and mixing.

This kit offers simultaneous quantitative and qualitative assessment of total human and human male DNA in a single, highly sensitive real-time PCR reaction. Here are the key features and benefits of the Quantifiler Trio kit:

Highly accurate

• Determines the ratio of human male to female DNA, even with minute male and excess female DNA (1:4000 or greater)
• Includes a pooled, human genomic DNA standard for improved stability and homogeneity

Highly sensitive

• <1 pg/μL limit of detection
• Quantifies DNA concentrations from 0.005 ng/μL to >50 ng/μL, enabling a broad range of concentrations
• Flexible protocols for higher concentration samples

Highly specific

• Detects human and higher primate DNA
• Three human-specific target loci: small autosomal, large autosomal, and a Y-chromosome target

Highly informative

• Quantifies DNA from various sources, including buccal swabs, blood, semen, tissue, and other human DNA sources
• Guides selection of the optimal AmpFLSTR PCR Amplification Kit (autosomal, Y-STR, or miniSTR)
• Quality Index improves prediction of STR amplification performance and estimation of DNA quantity in degraded samples
• Enhanced inhibition detects the presence of PCR inhibitors, suggesting additional purification or the need for a mini STR kit

Highly reliable

• Uses gold standard TaqMan real-time PCR technology widely adopted in forensic laboratories
• Utilizes multiple-copy target loci for improved detection sensitivity
• Real-time PCR has the widest dynamic range for accurate quantification of low-level samples

Highly efficient

• Dual quantification system minimizes sample consumption and streamlines workflow
• Optimized chemistry for more efficient multiplexing, faster PCR cycle times, and better inhibitor tolerance
• Results in approximately one hour

A highly informative workflow tool

• Improved performance to match newer STR kits designed for challenging samples
• Validated for use with the HID NIMBUS Presto QNA and HID NIMBUS QNA systems for quantification plate setup
• Packaging features to minimize reagent contamination and accommodate automated platforms
• Compatible with commonly used extraction technologies for easy integration into current lab workflows
• Enables improved and integrated workflow for more effective processing of forensic casework samples
• Fully automatable with most liquid handling robotic platforms