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Citations & References (33)
Invitrogen™
ATTO-TAG™ CBQCA Derivatization Reagent (CBQCA; 3-(4-Carboxybenzoyl)quinoline-2-Carboxaldehyde)
ATTO-TAG™ CBQCA誘導体化試薬（CBQCA; 3-（4カルボキシベンゾイル）キノリン- 2-カルボキシアルデヒド）は、第1級アミンと特異的に反応し、電気泳動方式またはクロマトグラフ方式で解析できるコンシュゲートを形成します詳細を見る
| 製品番号（カタログ番号） | 数量 |
|---|---|
| A6222 または、製品番号A-6222 | 10 mg |
製品番号（カタログ番号） A6222
または、製品番号A-6222
価格（JPY）お問い合わせください ›
90,900
Each
数量:
10 mg
ATTO-TAG™ CBQCA誘導体化試薬（CBQCA; 3-（4カルボキシベンゾイル）キノリン- 2-カルボキシアルデヒド）は、第1級アミンと特異的に反応し、電気泳動方式またはクロマトグラフ方式で解析できるコンシュゲートを形成します。CBQCAの生成物は、450 nmで最大励起するか、He-Cdレーザーの442 nmスペクトル線によって最大励起し、最大発光は約550 nmです。キャピラリーゾーン電気泳動（CZE）では、ATTO-TAG™ CBQCAコンシュゲートの検出感度はアトモル範囲（10～18モル）である必要があります。高感度であり、バックグラウンドの懸念がなく、長波長興奮性を持つため、これらの電位試薬は研究者、診断、法医学での用途に適しています。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
化学反応性アミン
使用対象（アプリケーション）細胞の生存率、増殖、および機能。タンパク質電気泳動およびウェスタンブロッティング
標識または色素ATTO-TAG CBQCA
数量10 mg
反応性部分アルデヒド
出荷条件室温
ターゲットPrimary Amine
形状固体
製品ラインATTO-TAG
タイプATTO-TAG™ CBQCA誘導体化試薬
Unit SizeEach
組成および保存条件
フリーザー（-5～-30度）に保存し、遮光してください。
よくあるご質問（FAQ）
Can CBQCA (3-(4carboxybenzoyl) quinoline- 2-carboxaldehyde) be used to label reducing sugars?
Can NaCN be used instead of KCN in the CBQCA (3-(4carboxybenzoyl) quinoline- 2-carboxaldehyde) assay?
引用および参考文献 (33)
引用および参考文献
Abstract
CBQCA assay of primary amine losses during hemodialysis.
Journal:Clin Chim Acta
PubMed ID:11412827
Nitrogen loss during hemodialysis is an important issue since all steps taken to improve clearance of urea will also increase loss of amino acids and other normally recyclable sources of nitrogen. Most dialyzers exclude albumin and other proteins, which react well with dyes commonly employed to measure protein, but do
Derivatization in capillary electrophoresis.
Journal:J Chromatogr A
PubMed ID:9646486
'In recent years capillary electrophoresis (CE) has been developed into a versatile separation technique, next to gas and liquid chromatography (LC), well suited for the determination of a wide variety of e.g., pharmaceutical, biomedical and environmental samples. The main advantages of CE over chromatographic separation techniques are its simplicity and
Capillary electrophoresis with laser-induced fluorescence detection: a sensitive method for monitoring extracellular concentrations of amino acids in the periaqueductal grey matter.
Journal:J Neurosci Methods
PubMed ID:8815306
'The use of capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) for the analysis of microdialysate samples from the periaqueductal grey matter (PAG) of freely moving rats is described. By employing 3-(4-carboxybenzoyl)-2-quinoline-carboxaldehyde (CBQCA) as a derivatization agent, we simultaneously monitored the concentrations of 8 amino acids (arginine, glutamine, valine, gamma-amino-n-butyric acid
Mycothiol biosynthesis and metabolism. Cellular levels of potential intermediates in the biosynthesis and degradation of mycothiol in mycobacterium smegmatis.
Journal:J Biol Chem
PubMed ID:9804803
'Mycothiol (MSH; 1-D-myo-inosityl-2-(N-acetyl-L-cysteinyl)amido-2-deoxy-alpha-D- glucop yranoside (AcCys-GlcN-Ins)) is a novel thiol produced at millimolar levels by mycobacteria and other actinomycetes that do not make glutathione. We developed methods to determine the major components of MSH (AcCys, Cys-GlcN, AcCys-GlcN, Cys-GlcN-Ins, GlcN-Ins) in cell extracts. Mycobacterium smegmatis was shown to produce measurable levels
Identification of a recombinant synaptobrevin-thioredoxin fusion protein by capillary zone electrophoresis using laser-induced fluorescence detection.
Journal:J Chromatogr B Biomed Sci Appl
PubMed ID:9271130
'Capillary zone electrophoresis (CZE) was utilized to identify a synaptobrevin-thioredoxin fusion protein (TSB-51). TSB-51 is a substrate for cleavage by botulinum toxin B at the Q(76)-F(77) site. TSB-51 was derivatized with a fluorophore, CBQCA [3-(4-carboxy-benzoyl)-2-quinoline-carboxaldehyde], for 4 h at room temperature. Optimal conditions for CZE separation of the TSB-51-CBQCA complex