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Thermo Scientific™
EquiPhi29™ DNA Amplification Kit
The Thermo Scientific EquiPhi29 DNA Amplification Kit includes all required components for fast, sensitive, and efficient isothermal DNA amplification, which詳細を見る
製品番号（カタログ番号） A65393
価格（JPY）お問い合わせください ›
55,200
Each
数量:
100 Reactions
The Thermo Scientific EquiPhi29 DNA Amplification Kit includes all required components for fast, sensitive, and efficient isothermal DNA amplification, which includes rolling circle amplification (RCA) and multiple displacement amplification – whole genome amplification (MDA-WGA).
Features of the EquiPhi29 DNA Amplification Kit include:
• Fast DNA amplification in less than two hours
• Sensitivity from 1 fg of input DNA
• Maximal yield of 17 µg amplified DNA
• Compatibility with different types of sample input material (e.g., purified DNA, liquid media culture, agar plate colonies)
• Compatibility with various downstream applications such as DNA sequencing (Sanger sequencing, next-generation sequencing), digestion with restriction enzymes, cell-free DNA enrichment, and cell-free protein expression
• All required components for DNA amplification in RCA or MDA-WGA reactions
The kit contains EquPhi29 DNA Polymerase, a proprietary phi29 DNA polymerase mutant that possesses strong strand displacement activity and enables high thermostability, reaction speed, and product yield and low amplification bias. Also included is an optimized reaction buffer, exo-resistant random primer, pyrophosphatase, and dNTPs. The addition of pyrophosphatase to the reaction mixture enhances DNA amplification and facilitates sample handling.
研究用途にのみご使用ください。診断目的には使用できません。
仕様
フォーマットKit
最適反応温度42°C
反応時間Up to 2 hours
出荷条件Dry Ice
使用対象（アプリケーション）RCA, MDA-WGA
形状Liquid
ポリメラーゼEquiPhi29 DNA ポリメラーゼ
製品タイプDNA Amplification Kit
数量100 Reactions
研究カテゴリーNucleic Acid Analysis, In Vitro Transcription, Cell-free Protein Expression, In Situ Sequencing, SNP Detection
Unit SizeEach
組成および保存条件
• EquiPhi29 DNA Polymerase (0.1 mL at 10 U/μL)
• 10X EquiPhi29 DNA Polymerase Reaction Buffer (1 mL)
• 0.1 M DTT (0.25 mL)
• 500 μM Exo-Resistant Random Primer (0.2 mL)
• 10 mM dNTP Mix (0.2 mL)
• 0.1 U/μL Pyrophosphatase, inorganic (0.1 mL)
Store at -15 to -25°C.