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Invitrogen™
MEGAscript™ SP6 Transcription Kit
Novel transcription reaction conditions and Ambion's patented, high-yield technology allow the synthesis of 10–50 times the amount of RNA produced詳細を見る
| 製品番号（カタログ番号） | 製品タイプ | 数量 |
|---|---|---|
| AM1330 | SP6 In Vitro転写キット | 40反応 |
製品番号（カタログ番号） AM1330
価格（JPY）お問い合わせください ›
96,400
Each
製品タイプ:
SP6 In Vitro転写キット
数量:
40反応
Novel transcription reaction conditions and Ambion's patented, high-yield technology allow the synthesis of 10–50 times the amount of RNA produced by conventional transcription reactions. Each Ambion™ kit includes sufficient reagents for 40 reactions.
• Exclusive, ultrahigh yield technology is fast: 2 hour reaction time
• Amplifies aRNA for gene array analysis and other applications
• Efficiently incorporates many modified nucleotides
A typical 20 μl MEGAscript™ reaction with 1 μg of the pTRI-Xef-1alpha control template will yield over 100 μg of transcript. The versatility of the MEGAscript™ kit allows for manipulation of the reactions to include specialized reagents such as modified nucleotides, cap analog, or additional polymerase.
Accessory Products:
MessageAmp™ aRNA Amplification Kits are powered by MEGAscript™ technology and have been developed especially for the amplification of cRNA prior to array analysis. To purify in vitro transcription products from free nucleotides, buffer components, and enzymes, Ambion recommends using the MEGAclear™ Kit (SKU#AM1908) and the MEGAclear™-96 Kit (SKU#AM1909).
• Exclusive, ultrahigh yield technology is fast: 2 hour reaction time
• Amplifies aRNA for gene array analysis and other applications
• Efficiently incorporates many modified nucleotides
A typical 20 μl MEGAscript™ reaction with 1 μg of the pTRI-Xef-1alpha control template will yield over 100 μg of transcript. The versatility of the MEGAscript™ kit allows for manipulation of the reactions to include specialized reagents such as modified nucleotides, cap analog, or additional polymerase.
Accessory Products:
MessageAmp™ aRNA Amplification Kits are powered by MEGAscript™ technology and have been developed especially for the amplification of cRNA prior to array analysis. To purify in vitro transcription products from free nucleotides, buffer components, and enzymes, Ambion recommends using the MEGAclear™ Kit (SKU#AM1908) and the MEGAclear™-96 Kit (SKU#AM1909).
研究用途にのみご使用ください。診断目的には使用できません。
仕様
使用対象（アプリケーション）In Vitro転写、合成mRNA、mRNA、トランスフェクション、LNP、GENEart、線形化DNAプラスミド、導入、PCR産物
反応数40反応
製品ラインAmbion、MEGAscript
製品タイプSP6 In Vitro転写キット
プロモーターSP6
数量40反応
出荷条件ドライアイス
原料PCR産物、線形化DNAプラスミド
収量100 µg
最終産物タイプ合成mRNA, mRNA
フォーマットキット
サンプルタイプmRNA, 合成mRNA
Unit SizeEach
組成および保存条件
SP6酵素ミックス、10X反応バッファー、ATP溶液、CTP溶液、GTP溶液、UTP溶液、pTRI-Xef、TURBO DNase、酢酸アンモニウム停止溶液、塩化リチウム沈殿溶液、およびゲルローディングバッファーIIは、すべて–20℃で保管します。ヌクレアーゼフリーの水は、何度で保管してもかまいません。