RiboPure™ RNA Purification Kit

The Ambion™ RiboPure™ Kit is designed for rapid purification of high-quality RNA from tissue samples or cultured cells. The kit詳細を見る

製品番号（カタログ番号）	製品タイプ
AM1924	キット

製品番号（カタログ番号） AM1924

価格（JPY）

107,300

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製品タイプ:

The Ambion™ RiboPure™ Kit is designed for rapid purification of high-quality RNA from tissue samples or cultured cells. The kit combines TRI Reagent™ with glass fiber filter purification to yield exceptionally pure RNA, free of residual proteins and lipids. The kit includes sufficient reagents for 50 purifications from 1–100 mg of tissue or 0.1–20 x 10⁶ cultured cells each.

• Yields the highest purity RNA attainable
• Ideal for the most stringent downstream applications
• Handles problematic samples with ease
• Fast (less than 30 minutes) and easy procedure

RNA isolated using the RiboPure™ Kit is suitable for use in all common downstream applications, including cDNA synthesis, real-time and end-point RT-PCR, microarray analysis, northern blots, and RNase protection assays. The RiboPure™ protocol is especially suited for processing samples that are difficult to disrupt, or samples that have large amounts of ribonuclease or other contaminants. Samples are first homogenized in TRI Reagent™, a monophasic solution containing phenol and guanidine thiocyanate, to rapidly lyse cells and inactivate nucleases. Addition of bromochloropropane (BCP) results in separation of the homogenate into aqueous and organic phases. RNA partitions to the aqueous phase, while DNA and protein remain in the interphase and organic phase. The aqueous phase is removed and passed through a glass fiber filter that binds the RNA. The filter is washed, and the RNA is eluted. The procedure is compatible with tissues that have been stored in Ambion's RNAlater™. Total RNA yield is typically 100–500 μg per 100 mg of tissue, depending on the type of tissue.

研究用途にのみご使用ください。診断目的には使用できません。

溶出量100 μL

最終産物タイプトータルRNA

使用対象（アプリケーション）マイクロアレイ解析、リアルタイム定量PCR（qPCR）、逆転写酵素PCR（RT-PCR）、cDNAライブラリ構築、in Vitro翻訳、ヌクレアーゼプロテクションアッセイ、ノーザンブロッティング

高スループット適合性ハイスループット非対応（手動）

製品タイプキット

精製時間30分

数量50 Preps

出荷条件室温

出発物質量最大2 x 10^7個の細胞、最大100 mgの組織

収量500 µg

Isolation Technology有機抽出

サンプルタイプ哺乳類細胞, 組織（哺乳類）

Unit SizeEach

組成および保存条件

TRI Reagentは4℃で保存します。フィルターカートリッジ、コレクションチューブ（2 mL）、洗浄液濃縮液、溶出バッファーは室温で保管します。

よくあるご質問（FAQ）

I'm getting poor quality/poor purity of RNA after isolation with the RiboPure RNA Purification kit. What could be the cause of this?

Poor quality/poor purity RNA is typically due to the following:

- Partially degraded RNA: Use fresh tissue or cells, or use RNAlater Stabilization Solution if tissue cannot be frozen immediately.
- DNA contamination: If the sample contains organic solvents, strong buffers, or an alkaline solution, we recommend performing phase separation at 8 degrees C.
- A low A260/A280 ratio (<1.6): This indicates that an insufficient amount of the TRI Reagent was used. Incubation of the homogenate at room temperature for 5 minutes can help nucleoproteins dissociate from RNA. Phenol contamination could also cause a low absorbance ratio.

What is the column cut-off size for the RiboPure RNA Purification Kits?

The column cut-off size is 200 nt. Using a RiboPure kit is not appropriate for miRNA recovery. For miRNA isolation, we would suggest using a mirVana kit, the TRIzol Plus RNA Purification Kit, or the PureLink RNA Micro Kit.

What are the differences in technology between the PureLink RNA Mini Kit and RiboPure RNA Purification Kits?

The PureLink RNA Mini Kit provides rapid column-based purification of total RNA, without organic lysis (phenol/chloroform). You can obtain up to 1,000 µg of purified RNA from a single extraction. The RiboPure RNA Purification Kits combine a phenol/guanidine thiocyanate solution with a glass-fiber filter purification method.

What is the composition of the elution buffer included in the RiboPure RNA Purification Kit (Cat. No. AM1924)?

The elution buffer composition is proprietary.