Ambion™ Poly(A) Polymerase catalyzes the addition of adenosine to the 3' end of RNA in a sequence-independent fashion.The enzyme can be used to append radiolabeled ATP to the 3' end of RNA molecules to generate labeled RNAs.One tube containing 80 U (2 U/ µL), 5X Poly(A) Polymerase Buffer, and 25 mM MnCl2 are provided.The enzyme can be used in conjunction with terminal transferase, reverse transcriptase, and thermostable polymerase to clone RNAs of unknown sequence.
Unit Definition: One unit is the amount of enzyme required to incorporate 1 nmol of AMP into acid-insoluble material in 10 min at 37°C.
研究用にのみ使用できます。診断用には使用いただけません。
仕様
濃度5X
数量80 units, 2 μL
出荷条件ドライアイス
電子線源RNA
ポリメラーゼポリ(A)RNAポリメラーゼ
製品ラインAmbion
Unit SizeEach
組成および保存条件
Poly(A) Polymerase, 5X Poly(A) Polymerase Buffer, and 25 mM MnCl2 should be stored at –20°C.
よくあるご質問(FAQ)
Is the Poly(A) Polymerase (cloned) (Cat. No. AM2030) from E. coli?
PolyA polymerase (cloned) is purified from an E. coli strain overexpressing E. coli Poly(A) Polymerase I. Here is the link to the product information sheet: https://assets.thermofisher.com/TFS-Assets/LSG/manuals/4393885_PlyAPlymrseCln_PI.pdf