Applied Biosystems 7900HTの384-well blockで、FastモードのTaqMan Real-Time PCRを行う場合、User Bulletin(P/N 4369584) " Performing Fast Gene Quantitation with 384-Well Plates"に操作の詳細な指示がまとめられていますので、参照してください。
How do I isolate DNA from single bacterial colonies before PCR?
Scrape a single colony from the surface of an agar plate and transfer to 12 µL of SCL solution [10 mM Tris-HCl (pH 7.5), 1 mM EDTA, 50 µg/mL proteinase K] and incubate for 15 min at 55 degrees C. Inactivate proteinase K for 15 min at 80 degrees C and add 20 µL of deionized water. Centrifuge at 12,000 x g for 3 min. Transfer the supernatant to a new tube.
I received Recombinant Proteinase K Solution (Cat. No. AM2546, AM2548) on Friday and it sat over the weekend and thawed. Is it still usable?
