This high-purity Ambion™ enzyme is a mix of cloned RNA polymerase and RNase inhibitor.It has a high specificity for its promoter, transcribing large amounts of RNA with no cross talk between promoters.It is supplied in one tube containing 30,000 U at a concentration of 200 U/ µL. The enzyme incorporates radiolabeled nucleotides along with many modified nucleotides.RNase阻害剤を添加することで、合成されたRNAの酵素分解に対する幅広いスペクトル保護が可能になり、ブロットおよびin situハイブリダイゼーションプローブ、RPA分析、in vitro翻訳、およびアンチセンスRNAのRNA合成に最適です。.
Can I substitute the enzyme mix in the MEGAscript T7 Transcription Kit (Cat. No. AM1333) with T7 RNA Polymerase-Plus Enzyme Mix (Cat. No. AM2718)?
No, we do not recommend substituting the enzyme mix in the MEGAscript T7 Transcription Kit (Cat. No. AM1333) with T7 RNA Polymerase-Plus Enzyme Mix (Cat. No. AM2718).