Guanidine Thiocyanate

Guanidine Thiocyanate is an ultrapure, molecular biology grade reagent.It is free of detectable nuclease and protease activity and is a詳細を見る

製品番号（カタログ番号）	数量
AM9422	500 g

製品番号（カタログ番号） AM9422

価格（JPY）

63,400

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500 g

Guanidine Thiocyanate is an ultrapure, molecular biology grade reagent.It is free of detectable nuclease and protease activity and is a strong protein denaturant, as both the guanidinium cation and the thiocyanate anion are chaotropic agents.It is provided in one bottle containing 500 g. It is recommended for isolation of RNA, especially for tissues such as pancreas with high levels of RNase activity.In solution, it is known as guanidinium thiocyanate.Guanidine Thiocyanate is thoroughly tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.

Specifications:
Appearance: White crystals Molecular Weight: 118.16純度:
>99%安定性：Unlimited under dry ambient conditions.Keep in dark container.

研究用にのみ使用できます。診断用には使用いただけません。

使用対象（アプリケーション）RNA抽出

製品ラインAmbion

製品タイプGuanidine Isothiocyanate

純度>99%

数量500 g

出荷条件室温

Unit SizeEach

組成および保存条件

室温保存。

よくあるご質問（FAQ）

What salts can be used to precipitate total RNA? What are the advantages of each? Do they require ethanol?

The three salts that can be used are:

- Guanidine thiocyanate, which requires ethanol. Guanidine thiocyanate is a common agent used for isolating RNA and we recommend it especially for tissues high in ribonuclease activity, such as the pancreas or spleen.
- Ammonium acetate, which requires ethanol. Ammonium acetate is useful when reducing coprecipitation of unwanted dNTPs and oligosaccharides. However, it should not be used when the nucleic acid will be phosphorylated using T4 polynucleotide kinase, since this enzyme is inhibited by ammonium ions.
- Lithium chloride, which does not require ethanol. LiCl is very effective in precipitating RNA but does not efficiently precipitate protein or DNA. It also does not precipitate unincorporated nucleotides.