Linear Acrylamide (5 mg/ml) (1 ml Tube)

Ambion™ Linear Acrylamide (5 mg/mL) is for use as a nucleic acid coprecipitant and is ideal for PCR and RT-PCR reactions since small amounts of contaminating nucleic acids present in other carriers could be amplified.各1 mL入りチューブ5本での提供です。

•Ideal for RT-PCR
• Increases pellet mass
• Quantitative recovery of low concentrations (ng/mL) of nucleic acid
• Prevents pellet loss in nuclease protection assays

What is a Coprecipitant?
Coprecipitants are inert substances used to aid recovery of nucleic acids during alcohol precipitations.大量の核酸を沈殿させるのにも使用できますが、希薄な溶液中の少量の核酸を定量的に回収するためには必須です。多くの場合、このような分子の使用は他の理由なく望ましいことですが、遠心分離後に沈殿した沈殿物を視覚化することが望ましいことがあります。Linear acrylamide offers advantages over other coprecipitants in that it is chemically synthesized and not treated with reagents (e.g. proteases) derived from biological sources.Therefore, it is not contaminated with biological material making it ideal for use upstream of RT-PCR.It has been shown to precipitate picogram amounts of DNA fragments larger than 20 base pairs while failing to precipitate shorter fragments and free nucleotides.Linear acrylamide is useful for separating reaction products from unincorporated nucleotides and from most oligonucleotide primers.It should be used at a final working concentration of 10–20 µg/mL.Linear acrylamide will not interfere with A_260/280 readings.