Sheared Salmon Sperm DNA is a very effective blocking agent when used in Northern prehybridization and hybridization buffers at a concentration of 100–200 µg/mL.
Using this product This sheared salmon sperm DNA has been treated with Proteinase K to remove any contaminating nucleases, followed by organic extraction with phenol:chloroform and ethanol precipitation.Salmon Sperm DNA is rigorously tested for RNase and DNase contamination and is suspended in nuclease-free water in a 1 mL tube at a concentration of 10 mg/mL (1 mL per tube).
研究用にのみ使用できます。診断用には使用いただけません。
仕様
使用対象(アプリケーション)ノーザンハイブリダイゼーション
製品ラインAmbion
製品タイプSalmon Sperm DNA
数量10 x 10 mg tubes
出荷条件ドライアイス
濃度10 mg/mL
Unit SizeEach
組成および保存条件
Store pure solution at –20°C
よくあるご質問(FAQ)
How many freeze thaw cycles do you recommend for Invitrogen, Salmon Sperm DNA, sheared (Cat. No. AM9680)?
We do not have specific guidance on the number of freeze thaws for Salmon Sperm DNA, but it can be frozen and thawed multiple times.
Do you have an alternative for Salmon Sperm DNA, sheared (10 mg/mL) (Cat. No. AM9680) to use as a spike-in for standard curve preparation for ssDNA?
You can use Yeast tRNA (10 mg/mL) (Cat. No. AM7119). When used at a final concentration of 10–20 µg/mL, yeast tRNA is an effective coprecipitant to aid in recovery of small amounts of nucleic acids.For more information, see the following link.