Luminex™ 200™ Instrument System

Each protein detected requires a different bead region to differentiate between analytes. The Luminex 200 Instrument System, Luminex FLEXMAP3D Instrument System and Luminex xMAP INTELLIFLEX System are outfitted with two lasers. The first laser is a red diode laser that classifies beads (region) based on color and hence identifies which protein is being measured. The second laser is a green YAG laser that quantitates reporter fluorophore (R-Phycoerythin, RPE) associated with the bead surface and determines the amount of the protein that is bound to the bead. The beads pass through the system at a rate of several thousand per second, excited first by the red laser and then the green laser. The results obtained show protein identity and the intensity of the signal, respectively. The intensity of the signal is directly proportional to the concentration of the analyte captured in the sample. Therefore, by monitoring the spectral properties of the beads and the amount of associated RPE fluorescence, the concentration of one or more proteins can be determined.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

We recommend using a Bead Region setting of 78.

ProcartaPlex Platinum Assay Buffer for Plasma Samples is designed to be used with the ProcartaPlex Platinum assay format. Currently, this format has been validated for human samples only. Other species have not been tested.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

ProcartaPlex Platinum Assay Buffer for Plasma Samples is designed to be used with the ProcartaPlex Platinum assay format. It is used for dilution of plasma samples and gives spike- and dilution-recovery results in the range of 70-130%. ProcartaPlex Platinum Assay Buffer for Plasma Samples is included in our Platinum ProcartaPlex Human Basic Kit for Plasma Samples (Cat.No. EPXP010-10421-901) and off-the-shelf Platinum panels. It is also offered as a stand-alone item (Cat. No. EPXP-11112-000).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

xMAP Sheath Concentrate PLUS, RUO (Cat. No. 4050023) is a 20X concentrated version of xMAP Sheath Fluid Plus (Cat. No. 4050021). It needs to be diluted before use.

xMAP Sheath Concentrate PLUS, RUO (Cat. No. 4050023) is a reformulation of xMAP Sheath Fluid Concentrate (Cat. No. 4050018). The formulation is updated from a single preservative to a mixture of three preservatives to allow for greater transit stability and to reduce the likelihood of future acquired microbial resistance.