Aluora™ Rainbow FluoCells™ Prepared Slide

This 9-plex fluorescent prepared slide is an axial section of rat kidney mounted with ProLong™ Glass and labeled with 8 Aluora™ dyes and DAPI. The labeling facilitates identification of kidney regions and is optimized for spectral unmixing, image acquisition, instrument QC, and data analysis.

The Aluora™ FluoCells™ kidney slide set is a mounted FFPE murine kidney control for spectral imaging. Slides are stained with covalent Aluora dyes plus DAPI and mounted in ProLong™ Glass to preserve signal. The set contains eight IHC targets plus DAPI for setup, calibration, teaching, and QC, with practical staining to show realistic channel separation and background. The kits include single-color controls, an unstained control, a 9-color rainbow slide, an H&E slide, and an Aluora MultiSpeck™ bead slide for alignment and illumination checks, also available as single components.

The Aluora MultiSpeck Spectral Calibration Bead Slide is a spectral fluorescence standard compatible with fluorescence microscopes and instruments that capture data from standard glass slides for optical alignment and illumination correction across a wide range of excitation sources.

The kit options are:

AS900 Aluora™ QC kit includes 9 single-color control slides, 1 unstained control, 1 rainbow multiplex slide, 1 H&E slide, plus 2 Aluora™ MultiSpeck™ spectral calibration bead slides; bead density supports XY optical alignment of objectives and within-objective illumination correction.

AS901KIT Aluora™ FluoCells™ kidney prepared slide set includes 9 single-color control slides, 1 unstained control, 1 rainbow slide, and 1 H&E slide (tissue-only format).

The single component options are:

AS903MPS Aluora™ Rainbow FluoCells™ prepared slide (rainbow multiplex slide only).

F36950 H&E FluoCells™ Prepared Slide – murine kidney (H&E slide only).

AS902MCS Aluora™ MultiSpeck™ spectral calibration bead slide (bead slide only).

The Aluora MultiSpeck Spectral Calibration Bead Slide has a uniform high density of ~4 μm-diameter microspheres that are compatible with a wide range of laser excitation sources (350 nm to 810 nm), LED excitation sources (375 nm to 730 nm), and standard fluorescent lamps. The beads emit fluorescence from 385 nm to 860 nm, making them ideal for optical alignment and illumination correction tasks.

Features and benefits

• Experimental design assistance—Family of fully mounted FFPE murine kidney control slides for spectral imaging instruments, supporting microscope setup, calibration, teaching, and ongoing quality control.
• Instrument and software optimization—Fluorescent tissue slides are stained with covalent Aluora™ dyes plus DAPI and mounted in ProLong™ Glass antifade mountant to help match refractive index and help maintain fluorescence signal.
• Data analysis training—Multiplex rainbow format combines eight IHC targets plus DAPI (9 colors total) in a single section for an end-to-end spectral unmixing example and image analysis practice.
• Built for calibration and alignment—Compatible with UV, violet, blue, yellow, green, red, deep red, far red, and NIR laser excitation sources, as well as standard fluorescent excitation lamps.
• Broad spectral emission range—Emits fluorescence from 385 nm to 860 nm, covering standard and long stokes-shifted fluorophores.
• High-density microspheres—Glass slide coated with uniform layer of ~4 μm-diameter microspheres in a clear 13-mm circle.

Versatile applications

• Workflow Training and QC—The Aluora labeled tissue slides support both experimental quality checks, and user training on image capture, spectral unmixing, and data analysis.
• Spectral responses of different filters and lenses—The proprietary fluorophore mixture in the microspheres mimics the spectral properties of a wide variety of dyes, allowing fluorescence measurement from almost any combination of excitation and emission filters.
• Illumination correction— Assists with objective illumination correction for evenness of fluorescence signal throughout the visible field.
• Image registration—Identifies x-y image registration differences when using multiple filters, enabling optical alignment compensation for filter variations.
• Multiband filters—The multispectral microspheres emit multiple colors, which can be observed through multiband filters, assisting with diverse color combinations for comprehensive spectral analysis.