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Citations & References (8)
Invitrogen™
Biotin Alkyne (PEG4 carboxamide-Propargyl Biotin)
ハプテン、ビオチンアジドは、銅触媒クリック反応を介して末端に反応します。その後、ビオチンはストレプトアビジン、アビジン、またはNeutrAvidin®ビオチン結合タンパク質で検出できます。詳細を見る
| 製品番号（カタログ番号） | 数量 |
|---|---|
| B10185 | 1 mg |
製品番号（カタログ番号） B10185
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45,500
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Ends: 27-Mar-2026
65,100割引額 19,600 (30%)
Each
数量:
1 mg
ハプテン、ビオチンアジドは、銅触媒クリック反応を介して末端に反応します。その後、ビオチンはストレプトアビジン、アビジン、またはNeutrAvidin®ビオチン結合タンパク質で検出できます。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
化学反応性アジド
フォーマット固体
標識または色素ビオチン
分子量528.66 Da
製品タイプビオチンアルキン
数量1 mg
反応基アルキン
反応性部分アルキン
出荷条件室温
溶解性DMSO（ジメチルスルホキシド）
色ビオチン
標識タイプビオチンおよびその他のハプテン
製品ラインMolecular Probes
Unit SizeEach
組成および保存条件
≦-20℃以下の湿気のない場所で保存してください。
引用および参考文献 (8)
引用および参考文献
Abstract
Identification of protein targets of 4-hydroxynonenal using click chemistry for ex vivo biotinylation of azido and alkynyl derivatives.
Journal:Chem Res Toxicol
PubMed ID:18232660
'Polyunsaturated fatty acids (PUFA) are primary targets of free radical damage during oxidative stress. Diffusible electrophilic alpha,beta-unsaturated aldehydes, such as 4-hydroxynonenal (HNE), have been shown to modify proteins that mediate cell signaling (e.g., IKK and Keap1) and alter gene expression pathways responsible for inducing antioxidant genes, heat shock proteins, and
Direct in-gel fluorescence detection and cellular imaging of O-GlcNAc-modified proteins.
Journal:J Am Chem Soc
PubMed ID:18683930
'We report an advanced chemoenzymatic labeling strategy for direct fluorescence detection of O-GlcNAc proteins in gels that facilitates proteomic studies and greatly extend the reach of existing technologies. These new tools also enable the expression and dynamics of O-GlcNAc modifications to be monitored by imaging in cells and tissues.
Comparative methods for analysis of protein covalent modification by electrophilic quinoids formed from xenobiotics.
Journal:Bioconjug Chem
PubMed ID:19301905
'Conjugation of biotin and fluorophore tags is useful for assaying covalent protein modification. Oxidative bioactivation of selective estrogen receptor modulators (SERMs) yields reactive quinoid electrophiles that covalently modify proteins, and bioactivation is associated with carcinogenic and chemopreventive effects. Identification of the protein targets of electrophilic metabolites is of general importance
HACE1 reduces oxidative stress and mutant Huntingtin toxicity by promoting the NRF2 response.
Journal:
PubMed ID:24516159
Oxidative stress plays a key role in late onset diseases including cancer and neurodegenerative diseases such as Huntington disease. Therefore, uncovering regulators of the antioxidant stress responses is important for understanding the course of these diseases. Indeed, the nuclear factor erythroid 2-related factor 2 (NRF2), a master regulator of the
O-linked N-acetylglucosamine modification on CCAAT enhancer-binding protein beta: role during adipocyte differentiation.
Journal:J Biol Chem
PubMed ID:19478079
CCAAT enhancer-binding protein (C/EBP)beta is a basic leucine zipper transcription factor family member, and can be phosphorylated, acetylated, and sumoylated. C/EBPbeta undergoes sequential phosphorylation during 3T3-L1 adipocyte differentiation. Phosphorylation on Thr(188) by MAPK or cyclin A/cdk2 primes the phosphorylations on Ser(184)/Thr(179) by GSK3beta, and these phosphorylations are required for the