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Citations & References (16)
Invitrogen™
Biotin-4-Fluorescein
ビオチン-4-フルオレセインは、利用可能なビオチン結合部位、またはアビジンやストレプトアビジンの濃度を、この分子がアビジンに結合したときに生じる強力な消光を通して定量化するために使用できます。詳細を見る
| 製品番号（カタログ番号） | 数量 |
|---|---|
| B10570 または、製品番号B-10570 | 5 mg |
製品番号（カタログ番号） B10570
または、製品番号B-10570
価格（JPY）お問い合わせください ›
61,000
Each
数量:
5 mg
ビオチン-4-フルオレセインは、利用可能なビオチン結合部位、またはアビジンやストレプトアビジンの濃度を、この分子がアビジンに結合したときに生じる強力な消光を通して定量化するために使用できます。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
発光523 nm
励起494 nm
標識または色素FITC(フルオレセイン)
分子量644.7
製品タイプビオチン-4-フルオレセイン
数量5 mg
出荷条件室温
Unit SizeEach
組成および保存条件
室温で保存し、光から保護します。
引用および参考文献 (16)
引用および参考文献
Abstract
Simultaneous multicolor fluorescence cross-correlation spectroscopy to detect higher order molecular interactions using single wavelength laser excitation.
Journal:Biophys J
PubMed ID:16632502
'Fluorescence cross-correlation spectroscopy is a powerful method for the study of molecular interactions and dynamics in solution and even in living cells. Usually, in the optical setup, either two laser beams have to be superimposed in their respective confocal volumes or two-photon excitation is used for a dual-color detection system.
Application of biotin-4-fluorescein in homogeneous fluorescence assays for avidin, streptavidin, and biotin or biotin derivatives.
Journal:Methods Mol Biol
PubMed ID:18287651
'Biotin-4-fluorescein (B4F) is a convenient molecular probe for (strept)avidin and for unlabeled biotin in homogeneous fluorescence assays. The primary standard is a 16 microM working solution of d-biotin which is used to titrate an aliquot of a (strept)avidin stock solution while monitoring the tryptophane fluorescence of (strept)avidin. This serves to
Patterning enzymes inside microfluidic channels via photoattachment chemistry.
Journal:Anal Chem
PubMed ID:15053641
'We have developed a general method for photopatterning well-defined patches of enzymes inside a microfluidic device at any location. First, a passivating protein layer was adsorbed to the walls and floor of a poly(dimethylsiloxane)/glass microchannel. The channel was then filled with an aqueous biotin-linked dye solution. Using an Ar+/Kr+ laser,
Fabrication of microarray of gel-immobilized compounds on a chip by copolymerization.
Journal:Biotechniques
PubMed ID:10489618
'The manufacturing of microchips containing oligonucleotides and proteins immobilized within gel pads, ranging in size from 10 x 10 to 100 x 100 microns, is described. The microchips are produced by photo- or persulfate-induced copolymerization of unsaturated derivatives of biomolecules with acrylamide-bisacrylamide mixture. Oligonucleotides containing 5''-allyl or 5''-butenediol units were
A streptavidin variant with slower biotin dissociation and increased mechanostability.
Journal:Nat Methods
PubMed ID:20383133
'Streptavidin binds biotin conjugates with exceptional stability but dissociation does occur, limiting its use in imaging, DNA amplification and nanotechnology. We identified a mutant streptavidin, traptavidin, with more than tenfold slower biotin dissociation, increased mechanical strength and improved thermostability; this resilience should enable diverse applications. FtsK, a motor protein important