Bis-ANS (4,4'-Dianilino-1,1'-Binaphthyl-5,5'-Disulfonic Acid, Dipotassium Salt)

Citations & References (233)

Invitrogen™

Bis-ANS (4,4'-Dianilino-1,1'-Binaphthyl-5,5'-Disulfonic Acid, Dipotassium Salt)

Bis-ANSは、タンパク質中の非極性キャビティーのプローブとして1-8-ANSよりも優れており、多くの場合、桁違いの高親和性で結合しています。アニリンホフタレンスルホン酸塩（ANS）は基本的に水中で蛍光を示さず、膜組織に結合したときにのみはっきりとした蛍光を示します。この特性により、プローブの水への暴露を修正するタンパク質フォールディング詳細を見る

製品番号（カタログ番号）	数量
B153	10 mg

製品番号（カタログ番号） B153

価格（JPY）

52,000

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10 mg

Bis-ANSは、タンパク質中の非極性キャビティーのプローブとして1-8-ANSよりも優れており、多くの場合、桁違いの高親和性で結合しています。アニリンホフタレンスルホン酸塩（ANS）は基本的に水中で蛍光を示さず、膜組織に結合したときにのみはっきりとした蛍光を示します。この特性により、プローブの水への暴露を修正するタンパク質フォールディング、立体構造変化、およびその他のプロセスに対する高感度な指標となります。

研究用にのみ使用できます。診断用には使用いただけません。

標識タイプその他の標識または色素

分子式C32H22K2N2O6S2

製品ラインMolecular Probes

数量10 mg

推奨保存方法室温で保存し、光から保護します。

出荷条件室温

細胞内局在細胞膜&脂質

物理的フォームSolid

製品タイプBis-ANS

Unit SizeEach

引用および参考文献 (233)

引用および参考文献

Abstract

Interaction of 1,1'-bi(4-anilino)naphthalene-5,5'-disulfonic acid with alpha-crystallin.

Authors:Sharma KK, Kaur H, Kumar GS, Kester K

Journal:J Biol Chem

PubMed ID:9535881

'The hydrophobic sites in alpha-crystallin were evaluated using a fluorescent probe 1,1''-bi(4-anilino)naphthalenesulfonic acid (bis-ANS). Approximately one binding site/subunit of alpha-crystallin at 25 degrees C was estimated by equilibrium binding and Scatchard analysis (Kd = 1.1 microM). Based on fluorescence titration, the dissociation constant was 0.95 microM. The number of bis-ANS ... More

Plurality of protein conformations of ribulose-1,5-bisphosphate carboxylase/oxygenase monomers probed by high pressure electrophoresis.

Authors:Erijman L, Paladini AA, Lorimer GH, Weber G

Journal:J Biol Chem

PubMed ID:7902356

'We used hydrostatic pressure in the range of 1 to 2 kbar, coupled with polyacrylamide gel electrophoresis, to investigate the properties of monomers of dimeric ribulose bisphosphate carboxylase/oxygenase. At temperatures below -5 degrees C or pressures above 1.5 kbar, only a diffuse band with low electrophoretic mobility was observed, which ... More

An operator-induced conformational change in the C-terminal domain of the lambda repressor.

Authors:Saha R, Banik U, Bandopadhyay S, Mandal NC, Bhattacharyya B, Roy S

Journal:J Biol Chem

PubMed ID:1532575

'4,4''-bis(1-anilino-8-naphthalenesulfonic acid (Bis-ANS), an environment-sensitive fluorescent probe for hydrophobic region of proteins, binds specifically to the C-terminal domain of lambda repressor. The binding is characterized by positive cooperativity, the magnitude of which is dependent on protein concentration in the concentration range where dimeric repressor aggregates to a tetramer. In this ... More

Exploiting new potential targets for anti-hepatitis B virus drugs.

Authors:Wen YM, Lin X, Ma ZM

Journal:Curr Drug Targets Infect Disord

PubMed ID:14529356

'Based on the recent studies of HBV strains with different replication efficiency, several new potential targets for anti-HBV replication have been presented. These include the viral and cellular regulatory factors associated with HBV replication and the process for encapsidation of viral genome and budding into endoplasmic reticulum (ER). A putative ... More

Detection of disulfide bonds in bovine brain tubulin and their role in protein folding and microtubule assembly in vitro: a novel disulfide detection approach.

Authors:Chaudhuri AR, Khan IA, Ludueña RF

Journal:Biochemistry

PubMed ID:11467944

'Cysteine residues in tubulin are actively involved in regulating ligand interactions and microtubule formation both in vivo and in vitro. These cysteine residues are sensitive reporters in determining the conformation of tubulin. Although some of the cysteines are critical in modulating drug binding and microtubule assembly, it is not clear ... More

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