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D-ビオチンは、ストレプトアビジン、アビジン、およびNeutrAvidin™ビオチン結合タンパク質と高い親和性で結合します。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
製品タイプビオチン(非コンジュゲート)
数量1 g
出荷条件室温
コンジュゲート非コンジュゲート
形状固体
製品ラインInvitrogen
Unit SizeEach
組成および保存条件
室温保存。
よくあるご質問（FAQ）
How should I resolubilize D-biotin (B20656)?
引用および参考文献 (19)
引用および参考文献
Abstract
High-performance liquid chromatographic determination of biotin in biological materials after crown ether-catalyzed fluorescence derivatization with panacyl bromide.
Journal:Anal Biochem
PubMed ID:1534471
This paper reports a high-performance liquid chromatographic (HPLC) technique to determine biotin in biological samples. Biotin and the internal standard dethiobiotin are converted into fluorescent derivatives by using panacyl bromide [p-(9-anthroyloxy)phenacyl bromide] as a fluorescence label. Biotin is extracted from biological tissue with trichloroacetic acid and the extract is purified
Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation.
Journal:Anal Biochem
PubMed ID:12419349
'The high-affinity binding of biotin to avidin, streptavidin, and related proteins has been exploited for decades. However, a disadvantage of the biotin/biotin-binding protein interaction is that it is essentially irreversible under physiological conditions. Desthiobiotin is a biotin analogue that binds less tightly to biotin-binding proteins and is easily displaced by
Diaminobiotin and desthiobiotin have biotin-like activities in Jurkat cells.
Journal:J Nutr
PubMed ID:12730407
'In mammals, biotin serves as a coenzyme for carboxylases such as propionyl-CoA carboxylase. The expression of genes encoding interleukin-2 (IL-2) and IL-2 receptor (IL-2R)gamma also depends on biotin. Biotin metabolites are structurally similar to biotin, and their concentrations in tissues are quantitatively important. Here, the hypothesis was tested that biotin
Cellular uptake of biotin: mechanisms and regulation.
Journal:J Nutr
PubMed ID:10064315
'This review describes our knowledge of biotin transport in the small intestine of humans and other mammals and presents recent findings in the area. Previous studies have shown that biotin transport across the brush border membrane of the small intestinal absorptive cells occurs via a carrier-mediated, Na+ gradient-dependent, electroneutral mechanism.
Biotin transport through the blood-brain barrier.
Journal:J Neurochem
PubMed ID:3098919
'The unidirectional influx of biotin across cerebral capillaries, the anatomical locus of the blood-brain barrier, was measured with an in situ rat brain perfusion technique employing [3H]biotin. Biotin was transported across the blood-brain barrier by a saturable system with a one-half saturation concentration of approximately 100 microM. The permeability-surface area