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Citations & References (15)
Invitrogen™
BrdUTP (5-Bromo-2'-Deoxyuridine 5'-Triphosphate), 10 mM in TE buffer
BrdUTPを末端デオキシヌクレオチド転位酵素（TdT）と組み合わせて使用することで、TUNELアッセイでアポトーシス細胞を標識できます。BrdUTPは、抗BrdU抗体で検出できます。詳細を見る
| 製品番号（カタログ番号） | 数量 |
|---|---|
| B21550 | 25 μL |
製品番号（カタログ番号） B21550
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Each
数量:
25 μL
BrdUTPを末端デオキシヌクレオチド転位酵素（TdT）と組み合わせて使用することで、TUNELアッセイでアポトーシス細胞を標識できます。BrdUTPは、抗BrdU抗体で検出できます。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
標識または色素その他の標識または色素
製品タイプBrdUTP
数量25 μL
出荷条件湿氷
濃度10 mM
Unit SizeEach
組成および保存条件
フリーザー（-5℃～-30℃）に保存し、遮光してください。
よくあるご質問（FAQ）
Can dU-containing DNA produced by amplification with dUTP be cut by restriction enzymes?
引用および参考文献 (15)
引用および参考文献
Abstract
Immunoseparation and immunodetection of nucleic acids labeled with halogenated nucleotides.
Journal:Exp Cell Res
PubMed ID:9260920
'A novel methodology for labeling, isolation, and detection of nucleic acids is described. Nucleic acid isolation is based on in vivo or in vitro incorporation of BrU or BrdU to either RNA or DNA, respectively, followed by immunoprecipitation of the labeled nucleic acid utilizing anti-BrdU MoAb, which crossreacts with BrU,
Purification and characterization of human topoisomerase I mutants.
Journal:Eur J Biochem
PubMed ID:8612607
'A system for rapid purification and characterization of eukaryotic topoisomerase-I mutants has been developed. The system utilizes six-histidine tagging of human topoisomerase I expressed in Saccharomyces cerevisiae to enable purification by nickel-affinity chromatography. Virtually homogenous mutant proteins are then tested for their ability to relax supercoiled DNA plasmids and their
Flow cytometric evaluation of apoptosis, necrosis and recovery when culturing monocytes.
Journal:J Immunol Methods
PubMed ID:11334964
'After developing and applying a method for cryopreserving monocytes, we found a substantial cell loss when culturing these cells. Monocytes were isolated from blood donors by density gradient centrifugation, purified by elutriation and cryopreserved. Thawed cells were cultured in ultra low attachment wells and studied with Annexin V, Propidium iodide,
A sensitive non-isotopic assay specific for HIV-1 associated reverse transcriptase.
Journal:J Virol Methods
PubMed ID:1713913
'A sensitive non-isotopic assay for specific detection of reverse transcriptase (RT) of the human immunodeficiency virus type 1 (HIV-1) is described using 5-bromo-2''-deoxyuridine triphosphate (BrdUTP) instead of tritiated thymidine triphosphate. After the RT reaction the template primer is degraded by alkaline hydrolysis. Single-stranded poly.(BrdU) is detected in an immunoenzymometric assay
A sensitive assay for the quantification of reverse transcriptase activity based on the use of carrier-bound template and non-radioactive-product detection, with special reference to human-immunodeficiency-virus isolation.
Journal:Biotechnol Appl Biochem
PubMed ID:8639277
'A non-radioactive 96-well microtitre plate reverse transcriptase (RT) assay, based on the use of covalently bound riboadenosine homopolymer in the wells and 5-bromodeoxyuridined 5''-triphosphate (BrdUTP) as dNTP, is described. The whole assay is performed in a single well, including the quantitative detection of incorporated BrdU, which is performed immunologically using