BacLight™ RedoxSensor™ Green Vitality Kit
<i>Bac</i>Light&trade; RedoxSensor&trade; Green Vitality Kit
Invitrogen™

BacLight™ RedoxSensor™ Green Vitality Kit

BacLight™ RedoxSensor™ Greenバイタリティキットに含まれる RedoxSensor™ Green試薬は、細菌還元酵素活性のインジケータです。この還元酵素活性は、電子伝達鎖官能基の変化や抗生物質処理後に起こる活性の変化を示す信頼できるマーカーです。RedoxSensor™ 緑色試薬は詳細を見る
製品番号(カタログ番号)数量
B34954200 kit
製品番号(カタログ番号) B34954
価格(JPY)
96,100
Each
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数量:
200 kit
BacLight™ RedoxSensor™ Greenバイタリティキットに含まれる RedoxSensor™ Green試薬は、細菌還元酵素活性のインジケータです。この還元酵素活性は、電子伝達鎖官能基の変化や抗生物質処理後に起こる活性の変化を示す信頼できるマーカーです。RedoxSensor™ 緑色試薬は、グラム陽性細菌とグラム陰性細菌の両方に浸透します。還元後、RedoxSensor™ 緑色試薬はホルムアルデヒド固定法に適合する安定した緑色蛍光シグナルを10分で生成します。

フローサイトメトリーのための微生物学アッセイに関する追加情報をご覧ください。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
細胞タイプ細菌
検出法蛍光
染色剤タイプRedoxSensor™ 緑色, PI
形状溶液
フォーマットチューブ、スライド
数量200 kit
出荷条件室温
溶解性DMSO(ジメチルスルホキシド)
EmissionPI:490⁄635、RedoxSensor™緑色:490⁄520
使用対象 (装置)蛍光顕微鏡, フローサイトメーター
製品ラインBacLight、RedoxSensor
製品タイプグリーンバイタリティキット
Unit SizeEach
組成および保存条件
1バイアルのRedoxSensor™緑色(200 μL、DMSO溶液1 mM)、1バイアルのヨウ化プロピジウム(300 μL、DMSO溶液20 mM)、1バイアルのアジ化ナトリウム(水溶液2 M中1 mL)、および1バイアルのCCCP(400 μL、DMSOに5 mM)が含まれます。

よくあるご質問(FAQ)

What bacterial parameters can I look at by flow cytometry?

You can stain bacteria with a general stain such as BacLight Green Bacterial Stain (Cat. No. B35000) or BacLight Red Bacterial Stain (Cat. No. B35001). You can look at gram character (Cat. No. L7005), cell viability (Cat. Nos. L7007, L7012, and L13152), cell count (Cat. Nos. L34856 and B7277), and cell vitality. Cell vitality can be measured by membrane potential (Cat. No. B34950) or by metabolism (Cat. Nos. B34954 and B34956).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the excitation/emission of the RedoxSensor Green reagent?

The excitation/emission of the RedoxSensor Green reagent, in reduced form, is 490/520 nm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (8)

引用および参考文献
Abstract
Respiration response imaging for real-time detection of microbial function at the single-cell level.
Authors:Konopka MC, Strovas TJ, Ojala DS, Chistoserdova L, Lidstrom ME, Kalyuzhnaya MG,
Journal:Appl Environ Microbiol
PubMed ID:21075887
'The ability to detect specific functions of uncultured microbial cells in complex natural communities remains one of the most difficult tasks of environmental microbiology. Here we present respiration response imaging (RRI) as a novel fluorescence microscopy-based approach for the identification of microbial function, such as the ability to use C(1) ... More
Extracellular reduction of uranium via Geobacter conductive pili as a protective cellular mechanism.
Authors:Cologgi DL, Lampa-Pastirk S, Speers AM, Kelly SD, Reguera G,
Journal:Proc Natl Acad Sci U S A
PubMed ID:21896750
'The in situ stimulation of Fe(III) oxide reduction by Geobacter bacteria leads to the concomitant precipitation of hexavalent uranium [U(VI)] from groundwater. Despite its promise for the bioremediation of uranium contaminants, the biological mechanism behind this reaction remains elusive. Because Fe(III) oxide reduction requires the expression of Geobacter''s conductive pili, ... More
Fullerene water suspension (nC60) exerts antibacterial effects via ROS-independent protein oxidation.
Authors:Lyon DY, Alvarez PJ,
Journal:Environ Sci Technol
PubMed ID:19031913
Buckminsterfullerene (C60) can form water suspensions (nC60) that exert toxic effects. While reactive oxygen species (ROS) generation has been implicated as the mechanism for mammalian cytotoxicity, we propose that nC60 exerts ROS-independent oxidative stress in bacteria, with evidence of protein oxidation, changes in cell membrane potential, and interruption of cellular ... More
Oxygen consumption rates of bacteria under nutrient-limited conditions.
Authors:Riedel TE, Berelson WM, Nealson KH, Finkel SE,
Journal:
PubMed ID:23770901
Many environments on Earth experience nutrient limitation and as a result have nongrowing or very slowly growing bacterial populations. To better understand bacterial respiration under environmentally relevant conditions, the effect of nutrient limitation on respiration rates of heterotrophic bacteria was measured. The oxygen consumption and population density of batch cultures ... More
In vitro susceptibility and cellular uptake for a new class of antimicrobial agents: dinuclear ruthenium(II) complexes.
Authors:Li F, Feterl M, Mulyana Y, Warner JM, Collins JG, Keene FR,
Journal:J Antimicrob Chemother
PubMed ID:22865383
To determine the in vitro susceptibility and cellular uptake for a series of dinuclear ruthenium(II) complexes [{Ru(phen)(2)}(2){µ-bb(n)}](4+) (Rubb(n)), and the mononuclear complexes [Ru(Me(4)phen)(3)](2+) and [Ru(phen)(2)(bb(7))](2+) against Staphylococcus aureus, methicillin-resistant S. aureus, Escherichia coli and Pseudomonas aeruginosa. The in vitro susceptibility was determined by MIC and MBC assays, and time-kill curve ... More