What bacterial parameters can I look at by flow cytometry?
You can stain bacteria with a general stain such as BacLight Green Bacterial Stain (Cat. No. B35000) or BacLight Red Bacterial Stain (Cat. No. B35001). You can look at gram character (Cat. No. L7005), cell viability (Cat. Nos. L7007, L7012, and L13152), cell count (Cat. Nos. L34856 and B7277), and cell vitality. Cell vitality can be measured by membrane potential (Cat. No. B34950) or by metabolism (Cat. Nos. B34954 and B34956).
'The ability to detect specific functions of uncultured microbial cells in complex natural communities remains one of the most difficult tasks of environmental microbiology. Here we present respiration response imaging (RRI) as a novel fluorescence microscopy-based approach for the identification of microbial function, such as the ability to use C(1) ... More
Extracellular reduction of uranium via Geobacter conductive pili as a protective cellular mechanism.
Authors:Cologgi DL, Lampa-Pastirk S, Speers AM, Kelly SD, Reguera G,
Journal:Proc Natl Acad Sci U S A
PubMed ID:21896750
'The in situ stimulation of Fe(III) oxide reduction by Geobacter bacteria leads to the concomitant precipitation of hexavalent uranium [U(VI)] from groundwater. Despite its promise for the bioremediation of uranium contaminants, the biological mechanism behind this reaction remains elusive. Because Fe(III) oxide reduction requires the expression of Geobacter''s conductive pili, ... More
Fullerene water suspension (nC60) exerts antibacterial effects via ROS-independent protein oxidation.
Authors:Lyon DY, Alvarez PJ,
Journal:Environ Sci Technol
PubMed ID:19031913
Buckminsterfullerene (C60) can form water suspensions (nC60) that exert toxic effects. While reactive oxygen species (ROS) generation has been implicated as the mechanism for mammalian cytotoxicity, we propose that nC60 exerts ROS-independent oxidative stress in bacteria, with evidence of protein oxidation, changes in cell membrane potential, and interruption of cellular ... More
Oxygen consumption rates of bacteria under nutrient-limited conditions.
Authors:Riedel TE, Berelson WM, Nealson KH, Finkel SE,
Journal:
PubMed ID:23770901
Many environments on Earth experience nutrient limitation and as a result have nongrowing or very slowly growing bacterial populations. To better understand bacterial respiration under environmentally relevant conditions, the effect of nutrient limitation on respiration rates of heterotrophic bacteria was measured. The oxygen consumption and population density of batch cultures ... More
In vitro susceptibility and cellular uptake for a new class of antimicrobial agents: dinuclear ruthenium(II) complexes.
Authors:Li F, Feterl M, Mulyana Y, Warner JM, Collins JG, Keene FR,
Journal:J Antimicrob Chemother
PubMed ID:22865383
To determine the in vitro susceptibility and cellular uptake for a series of dinuclear ruthenium(II) complexes [{Ru(phen)(2)}(2){µ-bb(n)}](4+) (Rubb(n)), and the mononuclear complexes [Ru(Me(4)phen)(3)](2+) and [Ru(phen)(2)(bb(7))](2+) against Staphylococcus aureus, methicillin-resistant S. aureus, Escherichia coli and Pseudomonas aeruginosa. The in vitro susceptibility was determined by MIC and MBC assays, and time-kill curve ... More