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DiSBAC2(3) (Bis-(1,3-Diethylthiobarbituric Acid)Trimethine Oxonol)
DiSBAC<sub>2</sub>(3) (Bis-(1,3-Diethylthiobarbituric Acid)Trimethine Oxonol)
Citations & References (120)
Invitrogen™

DiSBAC2(3) (Bis-(1,3-Diethylthiobarbituric Acid)Trimethine Oxonol)

低応答性の電位感受性プローブであるDiSBAC 2（3）は、脱分極した細胞や膜組織に入ってタンパク質や膜に結合します。これによって蛍光が強くなり、スペクトルにレッドシフトが認められます。脱分極が増加すると陰イオン色素の流入がさらに増加し、蛍光が増加します。逆に詳細を見る
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製品番号（カタログ番号）数量
B413100 mg
製品番号（カタログ番号） B413
価格（JPY）
47,800
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Ends: 27-Mar-2026
79,800
割引額 32,000 (40%)
Each
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数量:
100 mg
低応答性の電位感受性プローブであるDiSBAC 2（3）は、脱分極した細胞や膜組織に入ってタンパク質や膜に結合します。これによって蛍光が強くなり、スペクトルにレッドシフトが認められます。脱分極が増加すると陰イオン色素の流入がさらに増加し、蛍光が増加します。逆に、過分極は蛍光の減少によって示されます。このBis-oxonol型で生成される蛍光変化は電位に依存し、mVあたりの変化は通常∼1%です。また、このプローブの励起極大波長530 nm、発光極大波長560 nmです。DiBAC色素は、全体的に負電荷を有するためミトコンドリアから排除されます。このことから、血漿膜電位測定に対してはカルボシアン化色素よりも優れています。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
染色剤タイプ膜電位プローブ
数量100 mg
出荷条件室温
製品タイプDiSBAC2(3)
Unit SizeEach
組成および保存条件
室温で保存し、光から保護します。

よくあるご質問（FAQ）

I am seeing high background outside of my neuronal cells when using membrane potential indicators. What can I do to reduce background?

If you use our FluoVolt Membrane Potential Kit (Cat. No. F10488), the kit provides a background suppressor to reduce this problem. For other indicators, consider the use of BackDrop Background Suppressor (Cat no. R37603, B10511, and B10512).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between fast and slow-response membrane potential probes?

Molecules that change their structure in response to the surrounding electric field can function as fast-response probes for the detection of transient (millisecond) potential changes. Slow-response dyes function by entering depolarized cells and binding to proteins or membranes. Increased depolarization results in additional dye influx and an increase in fluorescence, while hyperpolarization is indicated by a decrease in fluorescence. Fast-response probes are commonly used to image electrical activity from intact heart tissues or measure membrane potential changes in response to pharmacological stimuli. Slow-responding probes are often used to explore mitochondrial function and cell viability.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What type of membrane potential indicators do you offer and how should I choose one for my experiment?

A membrane potential indicator selection guide can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-viability-and-regulation/ion-indicators/membrane-potential-indicators.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (120)

引用および参考文献
Abstract
Use of a membrane potential-sensitive probe to assess biological expression of the cystic fibrosis transmembrane conductance regulator.
Authors:Renier M, Tamanini A, Nicolis E, Rolfini R, Imler JL, Pavirani A, Cabrini G
Journal:Hum Gene Ther
PubMed ID:8590731
Cystic fibrosis is caused by defects in a chloride-transporting protein termed cystic fibrosis transmembrane conductance regulator (CFTR). This study presents an innovative procedure to evaluate expression of functional CFTR. The technique uses the potential-sensitive probe bis-(1,3-diethylthiobarbituric acid) trimethine oxonol or DiSBAC2(3), by single-cell fluorescence imaging. The DiSBAC2(3) method was first ... More
Expression of the voltage-gated sodium channel NaV1.5 in the macrophage late endosome regulates endosomal acidification.
Authors:Carrithers MD, Dib-Hajj S, Carrithers LM, Tokmoulina G, Pypaert M, Jonas EA, Waxman SG,
Journal:J Immunol
PubMed ID:17548620
'Voltage-gated sodium channels expressed on the plasma membrane activate rapidly in response to changes in membrane potential in cells with excitable membranes such as muscle and neurons. Macrophages also require rapid signaling mechanisms as the first line of defense against invasion by microorganisms. In this study, our goal was to ... More
Heat shock- and ethanol-induced ionic changes in C6 rat glioma cells determined by NMR and fluorescence spectroscopy.
Authors:Skrandies S, Bremer B, Pilatus U, Mayer A, Neuhaus-Steinmetz U, Rensing L
Journal:Brain Res
PubMed ID:9037501
'The effects of two different stressors, heat shock (HS; 44 degrees C, 20 min) and ethanol (1.2 M, 60 min), on ion content and membrane potential were investigated in C6 rat glioma cells. Both treatments were previously shown to induce the HS response [26]. Intracellular pH (pH(i)), sodium ion concentration ... More
Membrane potential modulates release of tumor necrosis factor in lipopolysaccharide-stimulated mouse macrophages.
Authors:Haslberger A, Romanin C, Koerber R
Journal:Mol Biol Cell
PubMed ID:1498365
'Lipopolysaccharide (LPS)-mediated synthesis of macrophage gene products such as tumor necrosis factor (TNF) is controlled by different signaling pathways. We investigated intracellular free Ca2+ (Ca2+ic) and the membrane potential as early cellular responses to LPS and their role in the synthesis and release of TNF. In peritoneal macrophages and in ... More
Selective modulation by cGMP of the K+ channel activated by speract.
Authors:Cook SP, Babcock DF
Journal:J Biol Chem
PubMed ID:7693667
'The egg peptide speract stimulates sperm guanylyl cyclase and presumably enhances fertilization, but the roles of cGMP in sperm responses are yet undetermined. Here we show that speract-induced accumulation of cGMP or cAMP is selectively enhanced by the phosphodiesterase inhibitors, 3-isobutyl-1-methylxanthine (IBMX) or papaverine, respectively. These inhibitors provided the unusual ... More
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