What gels can I use to separate native proteins?
The NativePAGE Invitrogen Bis-Tris Gel System is a pre-cast polyacrylamide mini gel system that provides a sensitive and high-resolution method for analyzing native membrane protein complexes, native soluble proteins, molecular mass estimations, and assessing purity of native proteins. It is based on the blue native polyacrylamide gel electrophoresis technique (BN PAGE) developed by Schagger and von Jagow.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
After transferring onto a nitrocellulose membrane, what is the best way to store the membrane overnight for probing the next day?
We recommend rinsing the membrane briefly in water, air drying and store it at room temperature in a ziplock bag. Do not place nitrocellulose in the freezer because it will shatter. Unlike PVDF, nitrocellulose membranes should never be pre-wetted in alcohol as it will cause them to shrivel.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
I would like to run a NativePAGE gel. Which of your protein standards should I use?
We recommend using the NativeMark Unstained Protein Standard, Cat. No. LC0725 for native gel electrophoresis with Tris-Glycine, NuPAGE Tris-Acetate or NativePAGE gels.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
Can I use the NativePAGE Sample and Running buffers with NuPAGE Bis-Tris gels?
We do not recommend using NativePAGE Sample and Running buffers with NuPAGE Bis-Tris gels. NuPAGE Invitrogen Bis-Tris gels are optimized for denaturing conditions and have an extremely low operating pH (pH 7.0), which makes it difficult for most proteins to migrate through them in a native state.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Can I use the NativePAGE Sample and Running buffers with Invitrogen Tris-Glycine gels or NuPAGE Tris-Acetate gels for native applications?
NativePAGE Sample Buffers and Running buffers were developed specifically for use with the NativePAGE Bis-Tris gels. We do not recommend using these buffers for native applications with any other gels, including NuPAGE Tris-Acetate or Invitrogen Tris-Glycine gels. For those gels, we recommend using the Invitrogen Tris-Glycine Native Sample and running buffers.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
