NativePAGE™ Sample Prep Kit
NativePAGE™ Sample Prep Kit
Invitrogen™

NativePAGE™ Sample Prep Kit

NativePAGE™サンプル調製キットには、ネイティブゲル電気泳動用のサンプル調製試薬が含まれています。このキットには、すぐに使用できる2つの界面活性剤溶液(10% DDM(n-ドデシル-β-D-マルトシド)と5%ジギトニン)が含まれており、サンプル調製中の疎水性タンパク質および膜タンパク質の溶解度を向上させます詳細を見る
製品番号(カタログ番号)数量
BN20081 kit
製品番号(カタログ番号) BN2008
価格(JPY)
38,100
Each
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数量:
1 kit
NativePAGE™サンプル調製キットには、ネイティブゲル電気泳動用のサンプル調製試薬が含まれています。このキットには、すぐに使用できる2つの界面活性剤溶液(10% DDM(n-ドデシル-β-D-マルトシド)と5%ジギトニン)が含まれており、サンプル調製中の疎水性タンパク質および膜タンパク質の溶解度を向上させます。

10%のDDM、5%のジギトニン、またはNativePAGE™サンプル調製キットで調製したサンプルは、NativePAGE™ Novex Bis-Tris Gelと互換性があり、ネイティブ電気泳動では分解能が向上し、ストリーキングが低減されます。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
バッファーサンプルローディングバッファー
ゲル適合性NativePAGE™ゲル
製品ラインNativePAGE
製品タイプPAGEサンプル調製キット
数量1 kit
出荷条件湿氷
ゲルタイプ天然ゲル
Unit SizeEach
組成および保存条件
内容:
• 1 mlの10% DDM
• 1 mlの5%ジギトニン
• 10 mlのNativePAGE™ 4X Sample Buffer
• 0.5 ml NativePAGE™ 5% G-250 Sample Additive。

キットは2℃~8℃で保存。

よくあるご質問(FAQ)

I would like to run a NativePAGE gel. Which of your protein standards should I use?

We recommend using the NativeMark Unstained Protein Standard, Cat. No. LC0725 for native gel electrophoresis with Tris-Glycine, NuPAGE Tris-Acetate or NativePAGE gels.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I use the NativePAGE Sample and Running buffers with NuPAGE Bis-Tris gels?

We do not recommend using NativePAGE Sample and Running buffers with NuPAGE Bis-Tris gels. NuPAGE Invitrogen Bis-Tris gels are optimized for denaturing conditions and have an extremely low operating pH (pH 7.0), which makes it difficult for most proteins to migrate through them in a native state.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the NativePAGE Sample and Running buffers with Invitrogen Tris-Glycine gels or NuPAGE Tris-Acetate gels for native applications?

NativePAGE Sample Buffers and Running buffers were developed specifically for use with the NativePAGE Bis-Tris gels. We do not recommend using these buffers for native applications with any other gels, including NuPAGE Tris-Acetate or Invitrogen Tris-Glycine gels. For those gels, we recommend using the Invitrogen Tris-Glycine Native Sample and running buffers.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the NativePAGE Sample and Running buffers with Invitrogen Tris-Glycine gels for native applications?

NativePAGE Sample Buffers and Running buffers were developed specifically for use with the NativePAGE Bis-Tris gels. We do not recommend using these buffers for native applications with any other gels, including Invitrogen Tris-Glycine gels. For those gels, we recommend using the Invitrogen Tris-Glycine Native Sample and running buffers.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the NativePAGE Sample and Running buffers with NuPAGE Tris-Acetate gels for native applications?

NativePAGE Sample Buffers and Running buffers were developed specifically for use with the NativePAGE Bis-Tris gels. We do not recommend using these buffers for native applications with any other gels, including NuPAGE Tris-Acetate gels. When running Tris-Acetate gels under native conditions, we recommend using the Invitrogen Tris-Glycine Native Sample and running buffers.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.