Human Epidermal Keratinocytes, neonatal (HEKn)

初代正常ヒト上皮ケラチン生成細胞、新生児（HEKn）は、新生児包皮から単離され、初代培養後に凍結保存されています。細胞の詳細：•性能試験済み — HKGS（HEKn用詳細を見る

製品番号（カタログ番号）	数量
C0015C	1 vial

製品番号（カタログ番号） C0015C

価格（JPY）

96,000

お問い合わせください ›

1 vial

初代正常ヒト上皮ケラチン生成細胞、新生児（HEKn）は、新生児包皮から単離され、初代培養後に凍結保存されています。

細胞の詳細：
•性能試験済み — HKGS（HEKn用）またはSupplement S7（HEKn-AOF用）を添加したEpiLife培地で培養した場合、30以上の集団倍加が得られます
• 70％以上の生存率が保証されています
• マイコプラズマ、細菌、酵母またはその他の真菌、B型肝炎、C型肝炎、およびHIV-1ウイルスについて試験済みです

各バイアルには0.5 x 10⁶を超える生細胞が含まれており、その細胞は、10% DMSOを含有する培地で初代培養段階の最後に凍結保存されています。

研究用にのみ使用できます。診断用には使用いただけません。

ドナー源シングルドナー

セル数≥500,000

製品ラインCascade Biologics

製品タイプヒト上皮ケラチノサイト

数量1 vial

試験済みB型肝炎（HBV）、C型肝炎（HCV）、無菌性試験（細菌&真菌）、マイコプラズマ、増殖性能アッセイ、HIV-1

Age新生児（14日以内）

細胞タイプケラチノサイト細胞

Culture Type接着細胞培養

形状凍結保存済み

Unit SizeEach

組成および保存条件

液体窒素で保存

よくあるご質問（FAQ）

I see large, flat pancake-looking cells in my keratinocyte culture. What are they?

These are senescent cells, and this is normal for adult keratinocyte culture. You always have a population of cells that are old and no longer proliferate. However younger cells, which are small in size should keep proliferating. When a culture gets older, you see more and more large cells, and the culture will eventually stop growing. With the right care an attention, the culture should yield at least 25 population doublings.

I see large, flat pancake-looking cells in my Human Keratinodyte culture. What are they?

These are senescent cells, and this is normal for adult keratinocyte culture. You always have a population of cells that are old and no longer proliferate. However younger cells, which are small in size, should keep proliferating. When a culture gets older, you see more and more large cells, and the culture will eventually stop growing. With the right care and attention, the culture should yield at least 25 population doublings.

What is the doubling time for Human Keratinodyte?

Each lot is tested for doubling time and the results are listed on the COA.

Can I specify a donor type for the primary cells you offer?

This information also appears on the COA.

What is the difference between basal cells or basal keratinocytes?

These are all the same. Sometimes Keratinocytes are referred to as basal cells or basal keratinocytes.

View all Human Epidermal Keratinocytes, neonatal (HEKn) FAQs

引用および参考文献 (1)

引用および参考文献

Abstract

High Sp1/Sp3 ratios in epithelial cells during epithelial differentiation and cellular transformation correlate with the activation of the HPV-16 promoter.

Authors:Apt D, Watts RM, Suske G, Bernard HU,

Journal:Virology

PubMed ID:8862423

Gene expression of human papillomavirus type 16 (HPV-16) and other HPV types is epithelial specific. Specificity is brought about by synergism between several different transcription factors that seem to occur ubiquitously but differ qualitatively and quantitatively between cells in which HPV genomes are transcriptionally active or inactive. Here, we report ... More