Primary human epidermal melanocytes isolated from adult skin, cryopreserved at the end of the secondary culture. These cells are isolated from lightly pigmented tissue.
Viability at least 70%
≥500,000 viable cells/vial
Capable of at least 16 population doublings
Tested for:
Mycoplasma: Not Detected
Hepatitis B: Not Detected
Hepatitis C: Not Detected
HIV-1: Not Detected
Bacteria, yeast, and other fungi: Not Detected
Positive for Mel-5 antigen in the fourth culture after thawing
Do you have ethnicity information for your human epidermal melanocyte donor lines?
We do provide age/sex/pigmentation/ethnicity on the Certificate of Analysis (COA).
What media do you use during the first passage of primary cells?
We use various media and supplements for the various primary cells. Details can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/primary-cell-culture.html).
How is the population doubling calculated for primary cells?
Cumulative population doublings are calculated from the seeding density and harvesting density from each culture level.
What are typical primary cell yields from tissue?
It varies a lot depending on the age of donor, size of tissues, locations, and conditions arrived to our facility. Sometime a small amount of tissue gives us many cells and sometimes a large amount of tissue gives us a very small lot. Typically, we can get 30-300 vials.
Are primary cells isolated from tissue using enzymatic digestion?
Yes, our primary cells are isolated from tissue using enzymatic digestion.