Primary human epidermal melanocytes isolated from neonatal foreskin, cryopreserved at the end of the secondary culture. These cells are isolated from moderately pigmented tissue.
Do you have ethnicity information for your human epidermal melanocyte donor lines?
We do provide age/sex/pigmentation/ethnicity on the Certificate of Analysis (COA).
How should melanocytes be frozen?
Do not use Synth-a-Freeze Cryopreservation Medium to cryopreserve melanocytes. We recommend using DMEM with 10% FBS and 10% DMSO, or the Recovery Cell Culture Freezing Medium.
What media do you use during the first passage of primary cells?
We use various media and supplements for the various primary cells. Details can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/primary-cell-culture.html).
How is the population doubling calculated for primary cells?
Cumulative population doublings are calculated from the seeding density and harvesting density from each culture level.
What are typical primary cell yields from tissue?
It varies a lot depending on the age of donor, size of tissues, locations, and conditions arrived to our facility. Sometime a small amount of tissue gives us many cells and sometimes a large amount of tissue gives us a very small lot. Typically, we can get 30-300 vials.