CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit

Citations & References (13)

Invitrogen™

CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit

Name: CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit
SKU: C10427

テクニカルサポートオーダーサポート

表示を変更

製品番号（カタログ番号）	数量
C10427	100アッセイ
C10740	20アッセイ

2 オプション

製品番号（カタログ番号） C10427

価格（JPY）

75,200

Each

お問い合わせください ›

数量:

100アッセイ

CellEvent™カスパーゼ-3/7 Greenフローサイトメトリーアッセイキットは、アポトーシスを起こしている細胞における活性化されたカスパーゼ-3およびカスパーゼ-7のフローサイトメトリー検出を可能にします。このキットは、新しい蛍光発生基質CellEvent™カスパーゼ-3/7 Green検出試薬およびSYTOX™ AADvanced™死細胞染色剤を含みます。

•Caspase-3/7には、活性化されたカスパーゼ-3およびカスパーゼ-7のための認識配列が含まれます
• 識別が容易—生細胞、死細胞、およびアポトーシスを起こしている細胞の集団を明確に識別します
• 迅速な分析—洗浄と固定は不要です
• マルチカラー対応—488 nmレーザーまたは他のレーザーで励起可能な他の色素と結合します

CellEvent™カスパーゼ-3/7 Green検出試薬は、核酸結合色素に結合した4アミノ酸ペプチド（DEVD）で構成される細胞透過性試薬です。アポトーシス中、カスパーゼ-3およびカスパーゼ-7タンパク質が活性化され、DEVDペプチドにエンコードされたカスパーゼ3/7認識配列を開裂できます。認識配列の開裂および試薬によるDNA結合は、アポトーシスを起こしている細胞に約511/533 nmの最大吸収／最大発光を持つ明るい蛍光シグナルを標識します。SYTOX™ AADvanced™死細胞染色剤と併用すると、アポトーシスを起こしている細胞を生細胞および壊死細胞から簡単に識別できます。

すべてのシステムでアポトーシスを定義する単一パラメータがないため、アポトーシスを確実に検出するために、複数の測定を組み合わせて使用することを強く推奨します。

研究用にのみ使用できます。診断用には使用いただけません。

仕様

励起／発光SYTOX AADvanced：546/647
CellEvent Caspase 3/7 Green Detection Reagent：511/533

フローサイトメーターレーザーライン488

使用対象（アプリケーション）フローサイトメトリー

使用対象 (装置)Attune™アコースティックフォーカシングサイトメーター、フローサイトメーター

標識タイプその他の標識または色素

標識または色素CellEvent™ Caspase 3/7 Green Detection Reagent、SYTOX AADvanced死細胞染色

製品ラインCellEvent

製品タイプCaspase Assay Kit

数量100アッセイ

出荷条件湿氷

溶解性DMSO (Dimethylsulfoxide)

検出法蛍光

フォーマットキット

Unit SizeEach

組成および保存条件

1バイアルのCellEvent™ Caspase 3/7 Green Detection Reagent、1バイアルのSYTOX AADvanced死細胞染色剤、1バイアルのDMSOを含みます。

すべてのバイアルは、-20℃以下で直立した状態で保存し、乾燥して遮光します。

よくあるご質問（FAQ）

I want to study apoptosis using an Annexin V conjugate, but with adherent cells via microscopy instead of flow cytometry. Can this be done?

It has been done, but we don‘t recommend it. Both healthy cells and apoptotic cells possess phosphatidylserine on the cell surface, which can be detected with Annexin V, but apoptotic cells have significantly more of it. You can easily tell the difference between these two populations with flow cytometry, because flow cytometers are more sensitive and have a higher throughput. But with a microscope, you cannot always tell the difference, especially for adherent cells. Instead, for microscopy, we recommend a different technique, such as detecting caspases with CellEvent Caspase Detection Reagents.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use CellEvent Caspase-3/7 Green Detection Reagent with fixed samples?

No, it must be applied to live cell populations.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

With the CellEvent Caspase-3/7 Green Detection Reagent, may I stain cells with nucleic acid stains?

Yes, you may use SYTOX AADvanced Dead Cell Stain that is included in the CellEvent Caspase-3/7 Green Ready Flow Reagent (Cat. No. R37167) and in the CellEvent Caspase-3/7 Green Flow Cytometry Assay Kit (Cat. No. C10427, C10740). You may also try using other nucleic acid stains in the red or far red channels for staining cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

With the CellEvent Caspase-3/7 Green Detection Reagent, may I multiplex with fluorescent immunolabeling?

Yes. You can apply surface labels or other reagents. We recommend applying the surface labels or other reagents first.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is the CellEvent Caspase-3/7 Green Detection Reagent fixable?

The CellEvent Caspase-3/7 Green Detection Reagent is not covalently attached to any cellular component and may be lost upon fixation. For flow cytometry applications, after applying the reagent, you should examine without washing or fixation. For imaging applications, the CellEvent product signal may be retained after fixation in 3.7% formaldehdye for 15 min.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit, 100 Assays FAQs

引用および参考文献 (13)

引用および参考文献

Abstract

Counteracting autophagy overcomes resistance to everolimus in mantle cell lymphoma.

Authors:Rosich L, Xargay-Torrent S, López-Guerra M, Campo E, Colomer D, Roué G,

Journal:Clin Cancer Res

PubMed ID:22879389

'Mantle cell lymphoma (MCL) is an aggressive B-lymphoid neoplasm with poor response to conventional chemotherapy and short survival. The phosphatidylinositol 3-kinase/Akt/mTOR survival pathway is constitutively activated in MCL cells, thereby making the mTOR inhibition an attractive therapeutic strategy. The first clinical studies of everolimus (RAD001), an mTOR inhibitor, in relapsed ... More

The cytotoxicity of bupivacaine, ropivacaine, and mepivacaine on human chondrocytes and cartilage.

Authors:Breu A, Rosenmeier K, Kujat R, Angele P, Zink W,

Journal:

PubMed ID:23749443

'Intraarticular injections of local anesthetics are frequently used as part of multimodal pain regimens. However, recent data suggest that local anesthetics affect chondrocyte viability. In this study, we assessed the chondrotoxic effects of mepivacaine, ropivacaine, and bupivacaine. We hypothesized that specific cytotoxic potencies directly correlate with analgesic potencies, and that ... More

Proteomic analysis reveals that pardaxin triggers apoptotic signaling pathways in human cervical carcinoma HeLa cells: cross talk among the UPR, c-Jun and ROS.

Authors:Huang TC, Chen JY,

Journal:Carcinogenesis

PubMed ID:23615400

Pardaxin, an antimicrobial peptide secreted by the Red Sea flatfish Pardachirus marmoratus, inhibits proliferation and induces apoptosis of human cancer cell lines. However, the underlying molecular mechanisms are only partially understood at present. In this study, we used proteomic approaches and network reconstruction to clarify the mechanism of pardaxin-induced apoptosis ... More

Salinomycin inhibits breast cancer progression via targeting HIF-1a/VEGF mediated tumor angiogenesis in vitro and in vivo.

Authors:Dewangan J, Srivastava S, Mishra S, Divakar A, Kumar S, Rath SK

Journal:Biochem Pharmacol

PubMed ID:31028743

'Cancer is a complex disease wherein cells begin to divideabnormally and spread into surrounding tissues. Angiogenesis plays a crucial role in tumor progression as it is required for sustained growth and metastasis, therefore targeting angiogenesis is a promising therapeutic approach for breast cancer management. Salinomycin (SAL) has been reported to ... More

Gel-based cell manipulation method for isolation and genotyping of single-adherent cells.

Authors:Negishi R, Iwata R, Tanaka T, Kisailus D, Maeda Y, Matsunaga T, Yoshino T

Journal:Analyst

PubMed ID:30302469

'Genetic analysis of single-cells is widely recognized as a powerful tool for understanding cellular heterogeneity and obtaining genetic information from rare populations. Recently, many kinds of single-cell isolation systems have been developed to facilitate single-cell genetic analysis. However, these systems mainly target non-adherent cells or cells in a cell suspension. ... More

View all CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit, 100 Assays citations