OPP(O-プロパルギル-ピューロマイシン)は、完全なメチオニン含有培地で新しく翻訳されたタンパク質に効率的に取り込まれる、ピューロマイシンのアルキンアナログです。Click-iT™ Plus OPP Alexa Fluor™タンパク質合成アッセイまたはAlexa Fluor™ピコリルアジドツールキットと併用することで、OPPは蛍光顕微鏡、ハイコンテントイメージング、またはフローサイトメトリーを用いたタンパク質合成の検出において、高速で高感度な非放射性のメソッドを提供します。
研究用途にのみご使用ください。診断目的には使用できません。
仕様
数量5 mg
試薬タイプ新生タンパク質標識試薬
使用対象(アプリケーション)蛍光顕微鏡 ), ハイコンテントイメージング, フローサイトメトリー
製品ラインOPP(O-プロパルギル-ピューロマイシン)
製品タイプOPP
Unit SizeEach
組成および保存条件
フリーザーで-5℃~-30℃に保存し、乾燥させます。
引用および参考文献 (8)
引用および参考文献
Abstract
Transcriptome analysis of a CHO cell line expressing a recombinant therapeutic protein treated with inducers of protein expression.
'The search for specific productivity (qP) determinants in Chinese hamster ovary (CHO) cells has been the focus of the biopharmaceutical cell line engineering efforts aimed at creating ' ... More
Suppression of the GTPase-activating protein RGS10 increases Rheb-GTP and mTOR signaling in ovarian cancer cells.
The regulator of G protein signaling 10 (RGS10) protein is a GTPase activating protein that accelerates the hydrolysis of GTP and therefore canonically inactivates G proteins, ultimately terminating signaling. Rheb is a small GTPase protein that shuttles between its GDP- and GTP-bound forms to activate mTOR. Since RGS10 suppression augments ... More
Nucleolar Enrichment of Brain Proteins with Critical Roles in Human Neurodevelopment.
Authors:Slomnicki LP, Malinowska A, Kistowski M, Palusinski A, Zheng JJ, Sepp M, Timmusk T, Dadlez M, Hetman M,
Journal:Mol Cell Proteomics
PubMed ID:27053602
'To study nucleolar involvement in brain development, the nuclear and nucleolar proteomes from the rat cerebral cortex at postnatal day 7 were analyzed using LC-MS/iTRAQ methodology. Data of the analysis are available via ProteomeXchange with identifier PXD002188. Among 504 candidate nucleolar proteins, the overrepresented gene ontology terms included such cellular ... More
The VHL short variant involves in protein quality control.
Authors:Liu Y, Yang H, Zuo F, Chen L,
Journal:Gene
PubMed ID:27196060
'The von Hippel-Lindau (VHL) is the most important and frequently mutated gene in human clear cell renal cell carcinoma (ccRCC). In contrast to its long counterpart, the internal translational variant of VHL protein (VHLs) is evolutionarily conserved. Herein we present evidence that VHLs associates with ribosome complex via interaction with ... More
Requirement of Neuronal Ribosome Synthesis for Growth and Maintenance of the Dendritic Tree.
Authors:Slomnicki LP, Pietrzak M, Vashishta A, Jones J, Lynch N, Elliot S, Poulos E, Malicote D, Morris BE, Hallgren J, Hetman M,
Journal:J Biol Chem
PubMed ID:26757818
'The nucleolus serves as a principal site of ribosome biogenesis but is also implicated in various non-ribosomal functions, including negative regulation of the pro-apoptotic transcription factor p53. Although disruption of the nucleolus may trigger the p53-dependent neuronal death, neurotoxic consequences of a selective impairment of ribosome production are unclear. Here, ... More