CellROX™ Green Flow Cytometry Assay Kit

Citations & References (12)

Invitrogen™

CellROX™ Green Flow Cytometry Assay Kit

CellROX™ Greenフローサイトメトリーアッセイキットは、生細胞中の活性酸素種（ROS）のフローサイトメトリー検出を可能にします。このキットには、新しい蛍光性 CellROX™ Green試薬、SYTOX™詳細を見る

製品番号（カタログ番号）	数量
C10492	100 assays

製品番号（カタログ番号） C10492

価格（JPY）

66,000

お問い合わせください ›

100 assays

CellROX™ Greenフローサイトメトリーアッセイキットは、生細胞中の活性酸素種（ROS）のフローサイトメトリー検出を可能にします。このキットには、新しい蛍光性 CellROX™ Green試薬、SYTOX™ Red死細胞染色剤、N- アセチルシステイン（抗酸化剤、ネガティブコントロール用）、およびtert-ブチルハイドロパーオキサイド溶液（TBHP、ROSの誘導剤）が含まれます。

すべてのCellROX™試薬とキットの選択ガイドをご覧ください。

CellROX™ Greenフローサイトメトリーアッセイキットの特徴：

•ROSの存在下で酸化されるフローサイトメトリー用に配合された蛍光プローブ
• マルチカラー対応—他のレーザーラインで励起された蛍光体とのオーバーラップを最小限に抑え、他の試薬とのマルチプレクシングを容易化
• シンプルなプロトコル—細胞は完全培地または他の適切な緩衝液で染色でき、血清フリー培地は不要

CellROX™ Green検出試薬は、細胞透過性および非蛍光性の試薬であり、還元状態の間は非常に弱い蛍光を発します。この試薬は酸化するとDNAと結合し、508／525 nmに最大吸収/発光を有し、核および細胞質に局在して強力な蛍光シグナルを示します。付属のSYTOX™ Red死細胞染色剤と併用すると、フローサイトメトリーで酸化ストレスを受けた細胞およびストレスを受けていない細胞を死細胞と確実に区別できます。

研究用にのみ使用できます。診断用には使用いただけません。

フォーマットチューブ

数量100 assays

Excitation/EmissionCellROX緑：508/525、SYTOX赤：640/658

Unit SizeEach

よくあるご質問（FAQ）

What are the fluorescence excitation/emission maxima for the CellROX Green Reagent provided in the CellROX Green Flow Cytometry Assay Kit?

The excitation/emission maxima for the CellROX Green Reagent are 508/525 nm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need a formaldehyde-fixable reactive oxygen species detection assay. Is H2 DCFDA fixable?

H₂DCFDA and similar derivatives are not fixable. The same goes for dihydroethidium and dihydrorhodamine. However, CellROX Deep Red and CellROX Green are retained for a limited time upon fixation with formaldehyde. CellROX Green may be retained upon subsequent Triton X-100 permeabilization. Avoid the use of any acetone or alcohol-based fixatives or fixatives that include alcohol, such as formalin.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can CellROX Green dye diluted in DMSO be subjected to freeze-thaw cycles?

CellROX Green dye diluted in a DMSO stock solution is stable for multiple freeze-thaw cycles, but it is not stable long-term in aqueous solution. Please note, every time the stock solution is opened, some of the dye can oxidize, which will increase background noise. DMSO readily absorbs moisture from the air, especially when cold, so be sure to completely thaw the stock solution to room temperature before opening and only open briefly. Water in the DMSO will gradually cause the dye to precipitate and come out of solution. Minimize the number of times you use a stock solution to several freeze-thaw cycles or make small aliquots to reduce the number of freeze-thaw cycles.

Find additional tips, troubleshooting help, and resources within our Flow Cytometry Support Center.

What is the difference between the CellROX Green Flow Cytometry Assay Kit (Cat. No. C10492) and CellROX Green Reagent, for oxidative stress detection (Cat. No. C10444)?

The main difference between the CellROX Green Flow Cytometry Assay Kit (Cat. No. C10492) and CellROX Green Reagent, for oxidative stress detection (Cat. No. C10444), is the suggested final working concentrations and application. The CellROX Green in the CellROX Green Flow Cytometry Assay Kit is specifically designed for flow cytometry analysis and is used at a lower concentration than what is required for imaging, as fluorescence-activated cell sorting (FACS) is a very sensitive detection system.

The standalone CellROX Green Reagent (Cat. No. C10444) is marketed for fluorescent microscopy and require working concentrations appropriate for imaging. This can typically be as much as 10-fold higher than the concentration recommended for flowcytometry. The standalone CellROX Green Reagent can also be used for flowcytometry; however, the working concentration will have to be optimized. For the flow cytometry quick reference, use the link below.

CellROX Flow Cytometry Assay Kit Quick Reference

Find additional tips, troubleshooting help, and resources within our Flow Cytometry Support Center.

What dyes can I use to detect reactive oxygen species (ROS) in my bacteria?

Many dyes that are used on mammalian cells have also been shown to be useful in bacterial cells. For example, CellROX Deep Red Reagent has been shown to work in B. subtilis (see Reference: http://www-brs.ub.ruhr-uni-bochum.de/netahtml/HSS/Diss/RaatschenNadja/diss.pdf). If you are interested in a particular dye, but are not sure if it will work on your bacteria, literature searches are the best way to check to see if it has been tested. If not, then it may be worth testing yourself.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all CellROX™ Green Flow Cytometry Assay Kit FAQs

引用および参考文献 (12)

引用および参考文献

Abstract

Using transcriptomics to improve butanol tolerance in Synechocystis sp. PCC 6803.

Authors:Anfelt J, Hallström B, Nielsen JB, Uhlén M, Hudson EP,

Journal:

PubMed ID:24056459

Cyanobacteria are emerging as promising hosts for production of advanced biofuels such as n-butanol and alkanes. However, cyanobacteria suffer from the same product inhibition problems that plague other microbial biofuel hosts. High concentrations of butanol severely reduce the growth and even low amounts can negatively affect metabolic processes. An understanding ... More

MTI-101 (Cyclized HYD1) Binds a CD44 Containing Complex and Induces Necrotic Cell Death in Multiple Myeloma.

Authors:Gebhard AW, Jain P, Nair RR, Emmons MF, Argilagos RF, Koomen JM, McLaughlin ML, Hazlehurst LA,

Journal:

PubMed ID:24048737

Our laboratory recently reported that treatment with the d-amino acid containing peptide HYD1 induces necrotic cell death in multiple myeloma cell lines. Because of the intriguing biological activity and promising in vivo activity of HYD1, we pursued strategies for increasing the therapeutic efficacy of the linear peptide. These efforts led ... More

Electrochemical Potential Gradient as a Quantitative in Vitro Test Platform for Cellular Oxidative Stress.

Authors:Bryant C, Atha D, Reipa V

Journal:Antioxidants (Basel)

PubMed ID:27409641

Oxidative stress in a biological system is often defined as a redox imbalance within cells or groups of cells within an organism. Reductive-oxidative (redox) imbalances in cellular systems have been implicated in several diseases, such as cancer. To better understand the redox environment within cellular systems, it is important to ... More

Production of Superoxide in Bacteria Is Stress- and Cell State-Dependent: A Gating-Optimized Flow Cytometry Method that Minimizes ROS Measurement Artifacts with Fluorescent Dyes.

Authors:McBee ME, Chionh YH, Sharaf ML, Ho P, Cai MW, Dedon PC

Journal:Front Microbiol

PubMed ID:28377755

The role of reactive oxygen species (ROS) in microbial metabolism and stress response has emerged as a major theme in microbiology and infectious disease. Reactive fluorescent dyes have the potential to advance the study of ROS in the complex intracellular environment, especially for high-content and high-throughput analyses. However, current dye-based ... More

Coral cell separation and isolation by fluorescence-activated cell sorting (FACS).

Authors:Rosental B, Kozhekbaeva Z, Fernhoff N, Tsai JM, Traylor-Knowles N

Journal:BMC Cell Biol

PubMed ID:28851289

Generalized methods for understanding the cell biology of non-model species are quite rare, yet very much needed. In order to address this issue, we have modified a technique traditionally used in the biomedical field for ecological and evolutionary research. Fluorescent activated cell sorting (FACS) is often used for sorting and ... More

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