Click-iT™ Plus EdU Pacific Blue™ Flow Cytometry Assay Kit

EdU はDNAに化学的に取り込まれています。 そのため、サンプルを固定後、PBSを加えて4℃で一晩保存することが可能です。また、クリック反応により、Alexa Fluor® azide はEdUに共有結合で結合します。 そのため、クリック反応後も、PBS中で 4℃で一晩保存することが可能です。

Click-iT EdU Imaging Kit では、反応バッファーに含まれていた銅イオンによって、GFPやR-PEが消光するなど、他の検出系との互換性の問題がありました。 Click-iT Plus EdU Imaging Kit では、銅イオンにキレート剤を付加することにより他の物質への影響を減少させました。 それに伴い、今までの Alexa Fluor azide を Alexa Fluor picolyl azide という物質に変更することで、クリック反応そのものの効率が落ちないようにしています。　Click-iT Plus EdU Flow Kit につきましても同様です。

Click reactions have several general characteristics: the reaction is efficient, no extreme temperatures or solvents are required, the reaction is complete within 30 minutes, the components of the reaction are bio-inert, and perhaps most importantly, no side reactions occur-the label and detection tags react selectively and specifically with one another. This final point is a key advantage of this powerful detection technique; it is possible to apply click chemistry-labeled molecules to complex biological samples and detect them with unprecedented sensitivity due to the extremely low background of the reaction.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

One may store the sample after fixation overnight in PBS at 4^oC. For longer storage (<1 week) , store in buffer with 1-2% formaldehyde or in formalin to limit microbial growth. If you use sodium azide as a microbial inhibitor, it must be completely removed prior to the Click-iT reaction.

Find additional tips, troubleshooting help, and resources within our Cell Viability, Proliferation, Cryopreservation, and Apoptosis Support Center.

We do not recommend using phalloidin conjugates for staining actin in combination with traditional Click-iT or Click-iT Plus reactions since phalloidin is extremely sensitive to the presence of copper.

For staining actin in combination with traditional Click-iT or Click-iT Plus reactions, we recommend using anti-α-actin antibodies for staining actin in the cytoskeleton. You can find a list of our actin antibodies here.

Another option would be to use the Click-iT Plus Alexa Fluor Picolyl Azide Toolkit (Cat. Nos. C10641, C10642, C10643). These Click-iT Plus toolkits provide Copper and Copper protectant separately which makes it easier to titrate the copper concentration to obtain optimal labeling with minimal copper-mediated damage. You may need to optimize the click reaction with the lowest possible concentration of copper and then perform the phalloidin staining.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.