Calcein AM, Cell-permeant Green and Blue Dyes

Citations & References (658)

Invitrogen™

Calcein AM, Cell-permeant Green and Blue Dyes

Name: Calcein AM, Cell-permeant Green and Blue Dyes
SKU: C3099

Calcein AMは細胞を透過させる色素で、ほとんどの真核細胞における細胞生存率の測定に使用できます。生細胞では、非蛍光カルセインAMは、細胞内エステラーゼによるアセトキシメチルエステルの加水分解後に緑色蛍光カルセインに変換されます。この色素は、1 mgの固体（C-1430詳細を見る

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製品番号（カタログ番号）	製品タイプ	数量	色
C3099	色素	1 mL	Green
C3100MP	色素	20 x 50 μg	Green
C1430	色素	1mg	Green
C1429	Calcein Blue AM	1 mg	Blue
C34852	色素	20 x 50 μg	Green
C481	カルセイン	100 mg	Green

6 オプション

製品番号（カタログ番号） C3099

価格（JPY）

83,200

Each

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製品タイプ:

色素

数量:

1 mL

色:

Green

Calcein AMは細胞を透過させる色素で、ほとんどの真核細胞における細胞生存率の測定に使用できます。生細胞では、非蛍光カルセインAMは、細胞内エステラーゼによるアセトキシメチルエステルの加水分解後に緑色蛍光カルセインに変換されます。この色素は、1 mgの固体（C-1430）としても使用でき、DMSO（C-3099）で再懸濁します。この色素のより長い波長バージョンについては、新しいCellTraceカルセイン赤-オレンジAM（C-34851）をご確認ください。

研究用にのみ使用できます。診断用には使用いただけません。

仕様

細胞透過性Cell-permeant

濃度1 mg⁄ml

染色剤タイプその他の標識または色素

形状Solution in DMSO

数量1 mL

試薬タイプCell Tracker Compounds、Cell Labeling Reagents

出荷条件室温

標的酵素Esterase

色Green

Emission517

Excitation Wavelength Range494 nm

使用対象（アプリケーション）Cell Tracing, Cell Tracker

使用対象 (装置)Fluorescence Microscope

製品タイプ色素

Unit SizeEach

組成および保存条件

フリーザー（-5℃～-30℃）に保存。

よくあるご質問（FAQ）

I would like a dye to load in live cells such that it will self-quench at a high concentration, but if the cell dies, the dye will be released and unquenched. Do you have anything like that?

Yes. This is commonly done with calcein AM or FDA (fluorescein diacetate). These dyes will not fluoresce until cleaved by esterases. After modification by esterases and at very high concentrations, they will self-quench. Upon disruption of the plasma membrane, or cell death, the dye will be released into the extracellular medium, and become unquenched. Concentration and incubation time must be optimized to obtain adequate quenching.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to load liposomes with calcein. Should I use the AM form or the non-AM form?

Calcein, AM requires esterase cleavage of the acetoxymethyl (AM) ester to become fluorescent. Liposomes don't have esterases unless specifically constructed to include the enzyme. The water-soluble, non-AM form of calcein (Cat. No. C481), does not require esterase cleavage to be fluorescent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to do a cell migration study for around 4 hours and need to fluorescently label the cells with a dye. What do you recommend?

Calcein, AM and FDA (fluorescein diaceate) are examples of some dyes used for this application. Since these dyes are not incorporated or covalently attached to any cellular components, they may have a short retention time as some cell types may actively efflux the dye out of the cells. The CellTracker and CellTrace dyes include either a mild thiol-reactive chloromethyl group or amine-reactive succinnimidyl ester group to allow for covalent binding to cellular components, providing for better retention. As with any reagent, one should empirically determine retention times for the cell type used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am doing a Live/Dead assay using Calcein, AM, for live cells and ethidium homodimer-1 for dead cells. Can I fix the cells after labeling and retain the staining?

This is not recommended. Neither Calcein nor ethidium homodimer-1 bind to any cellular components upon fixation. There is no guarantee that the dyes will be retained upon fixation or any subsequent wash steps. We recommend scoring for live and dead cells as soon as possible after staining.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need a general cytoplasmic stain that does not overlap with the GFP in my cells. What do you recommend?

Calcein AM, a green dye, is typically used as a general cytoplasmic stain, but not recommended with GFP-positive cells. For GFP-expressing cells there are other colors available: Calcein Blue AM, Calcein Violet AM, and Calcein Red-Orange AM. The retention time of these dyes in live cells is dependent upon the inherent properties of the cell.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Calcein AM, Cell-permeant Green and Blue Dyes FAQs

引用および参考文献 (658)

引用および参考文献

Abstract

Nitric oxide mediates natural polyphenol-induced Bcl-2 down-regulation and activation of cell death in metastatic B16 melanoma.

Authors:Ferrer P,Asensi M,Priego S,Benlloch M,Mena S,Ortega A,Obrador E,Esteve JM,Estrela JM

Journal:The Journal of biological chemistry

PubMed ID:17135264

Cloning and expression of murine sister of P-glycoprotein reveals a more discriminating transporter than MDR1/P-glycoprotein.

Authors:Lecureur V,Sun D,Hargrove P,Schuetz EG,Kim RB,Lan LB,Schuetz JD

Journal:Molecular pharmacology

PubMed ID:10617675

Deposition of laminin 5 by keratinocytes regulates integrin adhesion and signaling.

Authors:Nguyen BP,Gil SG,Carter WG

Journal:The Journal of biological chemistry

PubMed ID:10926936

Large-scale chemical dissection of mitochondrial function.

Authors:Wagner BK,Kitami T,Gilbert TJ,Peck D,Ramanathan A,Schreiber SL,Golub TR,Mootha VK

Journal:Nature biotechnology

PubMed ID:18297058

Mitochondrial oxidative phosphorylation (OXPHOS) is central to physiology and disease pathogenesis. To systematically investigate its activity and regulation, we performed a wide range of assays of OXPHOS physiology and nuclear and mitochondrial gene expression across 2490 chemical perturbations in muscle cells. Through mining of the resulting compendium, we discovered that: ... More

Modification of the cytoplasmic domain of influenza virus hemagglutinin affects enlargement of the fusion pore.

Authors:Kozerski C,Ponimaskin E,Schroth-Diez B,Schmidt MF,Herrmann A

Journal:Journal of virology

PubMed ID:10906206

The fusion activity of chimeras of influenza virus hemagglutinin (HA) (from A/fpv/Rostock/34; subtype H7) with the transmembrane domain (TM) and/or cytoplasmic tail (CT) either from the nonviral, nonfusogenic T-cell surface protein CD4 or from the fusogenic Sendai virus F-protein was studied. Wild-type or chimeric HA was expressed in CV-1 cells ... More

View all Calcein AM, Cell-permeant Green and Blue Dyes citations