CellTrace™ Calcein Red-Orange, AM - Special Packaging

Citations & References (11)

Invitrogen™

CellTrace™ Calcein Red-Orange, AM - Special Packaging

製品番号（カタログ番号）	数量
C34851	20 x 50 μg

製品番号（カタログ番号） C34851

価格（JPY）

93,900

お問い合わせください ›

20 x 50 μg

CellTraceカルセインレッド-オレンジAMは細胞透過性の色素で、ほとんどの真核細胞における細胞生存率の測定に使用できます。カルセインAM（C-1430、C-3099、C-3100）とは異なり、CellTraceカルセインレッド-オレンジAMは本質的に蛍光です。したがって、細胞によって取り込まれない色素からのバックグラウンド蛍光を最小限に抑えるために、追加の洗浄ステップが必要になる場合があります。ただし、CellTraceカルセインレッド-オレンジ（577/590 nmの最大励起波長／最大発光波長）は、無傷の細胞膜を持つ生細胞に十分に保持されているため、細胞トレーサーや細胞生存率のインジケーターとして有用です。

研究用にのみ使用できます。診断用には使用いただけません。

細胞透過性Cell-permeant

概要Calcein, Red-Orange, AM - Special Packaging

染色剤タイプその他の標識または色素

形状凍結乾燥

数量20 x 50 μg

試薬タイプCell Tracker Compounds、Cell Labeling Reagents

出荷条件室温

標的酵素Esterase

Emission577⁄590

Excitation Wavelength Range577 nm

使用対象（アプリケーション）Cell Tracing, Cell Tracker

使用対象 (装置)Fluorescence Microscope

製品ラインCellTrace

製品タイプ色素

Unit SizeEach

組成および保存条件

CellTrace™カルセインレッド-オレンジ（凍結乾燥粉末）のバイアル20本入りです。フリーザー（-5℃～-30℃）に保存し、遮光してください。

よくあるご質問（FAQ）

I need a general cytoplasmic stain that does not overlap with the GFP in my cells. What do you recommend?

Calcein AM, a green dye, is typically used as a general cytoplasmic stain, but not recommended with GFP-positive cells. For GFP-expressing cells there are other colors available: Calcein Blue AM, Calcein Violet AM, and Calcein Red-Orange AM. The retention time of these dyes in live cells is dependent upon the inherent properties of the cell.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How do I reconstitute CellTrace Calcein Red-Orange, AM (Cat. No. C34851)?

You can dissolve the dye using high-quality, anhydrous dimethylsulfoxide (DMSO) up to 1 -2 mg/mL. The acetoxymethyl ester (AM) moiety on CellTrace Calcein Red-Orange, AM is susceptible to hydrolysis when exposed to water absorbed by the DMSO. Once prepared, use the DMSO stock solutions of CellTrace Calcein Red-Orange, AM within a short time period. Aqueous working solutions containing the dye should be prepared fresh and used on the same day. The working principle of CellTrace Calcein Red-Orange, AM is very similar to Calcein, AM, cell-permeant dye (Cat. No. C1430). The protocol given in the product manual for Calcein, AM with an additional wash step is suitable. Wash cells with pre-warmed buffer (e.g. PBS, HBSS) to remove residual serum present in the culture medium, load cells with reagent in buffer, incubate from 15-45 minutes and then wash cells with medium (with or without serum). For further information please see the User Guide.

Find additional tips, troubleshooting help, and resources within our Cell Imaging Support Center.

I stained two populations of cells, one with CellTracker Green and the other with CellTracker Red, but it looks like there may be crossover of the red dye to the green cells. What is going on?

One possibility is that there is spectral bleedthrough between the dyes. Be sure to check the single-color samples by imaging the red cells in green and imaging the green cells in red, using the optimal imaging settings for the other color. If you see bleedthrough with these controls, then you will have to reduce the dye label concentration to reduce the brightness of the dyes, or choose dyes that are farther apart spectrally. If the issue isn’t bleedthrough, another possibility is that the cells were not adequately washed after staining, allowing some unincorporated dye to remain and label the other cells after they were introduced. Extending washes and wash times should help with this.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I stained my cells with Calcein, AM, but the signal went away after I fixed my cells. Why is this?

Calcein, AM diffuses into cells, the 'AM' moiety is cleaved by cellular esterases, and then the dye molecules are observed in the cytoplasm without binding to anything. This gives a 'whole cell' stain. It also means that the dyes are not crosslinked with aldehyde-based fixation and thus will be lost upon fixation. Additionally, any disruption of plasma membrane, such as with detergents or trypsinization, will lead to leakage of the dye from the cell.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I'm trying to stain my cells with CellTracker dyes or CFDA SE, but I'm not seeing much signal. What can I do?

First, make sure you aren’t staining in the presence of serum, since serum can have esterase activity that can prematurely cleave the AM group on these dyes, preventing entry into cells. After staining, it’s okay to return the cells to medium containing serum. After this, you can try increasing the concentration and label time to get a higher intensity.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all CellTrace™ Calcein Red-Orange, AM - Special Packaging FAQs

引用および参考文献 (11)

引用および参考文献

Abstract

T lymphocytes expressing a CD16 signaling receptor exert antibody-dependent cancer cell killing.

Authors:Kudo K, Imai C, Lorenzini P, Kamiya T, Kono K, Davidoff AM, Chng WJ, Campana D,

Journal:

PubMed ID:24197131

To expand applications for T-cell-based immunotherapy in cancer, we designed a receptor that binds the Fc portion of human immunoglobulins and delivers activation signals. The construct included the high-affinity CD16 (FCGR3A) V158 variant, CD8a hinge, and transmembrane domains, along with signaling domains from CD3? and 4-1BB (TNFRSF9), forming a chimeric ... More

H2O2-induced endothelial NO production contributes to vascular cell apoptosis and increased permeability in rat venules.

Authors:Zhou X, Yuan D, Wang M, He P,

Journal:Am J Physiol Heart Circ Physiol

PubMed ID:23086988

Although elevated levels of H(2)O(2) have been implicated to play important roles in the pathogenesis of various cardiovascular diseases, the underlying mechanisms remain unclear. This study aims to examine the effect of H(2)O(2) on endothelial nitric oxide (NO) production in intact venules, and elucidate the role and mechanisms of NO ... More

Programmed reduction of ABC transporter activity in sea urchin germline progenitors.

Authors:Campanale JP, Hamdoun A,

Journal:Development

PubMed ID:22274698

ATP-binding cassette (ABC) transporters protect embryos and stem cells from mutagens and pump morphogens that control cell fate and migration. In this study, we measured dynamics of ABC transporter activity during formation of sea urchin embryonic cells necessary for the production of gametes, termed the small micromeres. Unexpectedly, we found ... More

Connexin-43 upregulation in micrometastases and tumor vasculature and its role in tumor cell attachment to pulmonary endothelium.

Authors:Elzarrad MK, Haroon A, Willecke K, Dobrowolski R, Gillespie MN, Al-Mehdi AB,

Journal:BMC Med

PubMed ID:18647409

The modulation of gap junctional communication between tumor cells and between tumor and vascular endothelial cells during tumorigenesis and metastasis is complex. The notion of a role for loss of gap junctional intercellular communication in tumorigenesis and metastasis has been controversial. While some of the stages of tumorigenesis and metastasis, ... More

The cellular mechanisms of neuronal swelling underlying cytotoxic edema.

Authors:Rungta RL, Choi HB, Tyson JR, Malik A, Dissing-Olesen L, Lin PJ, Cain SM, Cullis PR, Snutch TP, MacVicar BA,

Journal:

PubMed ID:25910210

Cytotoxic brain edema triggered by neuronal swelling is the chief cause of mortality following brain trauma and cerebral infarct. Using fluorescence lifetime imaging to analyze contributions of intracellular ionic changes in brain slices, we find that intense Na(+) entry triggers a secondary increase in intracellular Cl(-) that is required for ... More

View all CellTrace™ Calcein Red-Orange, AM - Special Packaging citations