CyQUANT™ Cell Proliferation Assays

Citations & References (17)

Invitrogen™

CyQUANT™ Cell Proliferation Assays

Name: CyQUANT™ Cell Proliferation Assays
SKU: C35012

CyQUANT™ NF細胞増殖アッセイは、集団中の細胞をカウントし、マイクロプレートフォーマットで増殖を測定するための迅速かつ高感度な方法を提供します。CyQUANT™ NF細胞増殖アッセイの特長：•MTTやalamarBlue™アッセイよりも高感度• ウェルあたり100～20,000細胞の直線的検出範囲（96ウェルマイクロプレート）• アッセイは1時間で完了増殖を測定する迅速かつシンプルな方法CyQUANT™詳細を見る

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製品番号（カタログ番号）	細胞タイプ	数量
C35012	Direct Cell	100 Microplates
C35011	Direct Cell	10 Microplates
C35013	Direct Red Cell	10個のマイクロプレート
C35007	NF Cell	200 Assays
C7026	For cells in culture	1000 Assays
C35006	NF Cell	1000 Assays
C7027	Cell Lysis Buffer	50 mL

7 オプション

製品番号（カタログ番号） C35012

価格（JPY）

459,600

Each

お問い合わせください ›

細胞タイプ:

Direct Cell

数量:

100 Microplates

CyQUANT™ NF細胞増殖アッセイは、集団中の細胞をカウントし、マイクロプレートフォーマットで増殖を測定するための迅速かつ高感度な方法を提供します。

CyQUANT™ NF細胞増殖アッセイの特長：

•MTTやalamarBlue™アッセイよりも高感度
• ウェルあたり100～20,000細胞の直線的検出範囲（96ウェルマイクロプレート）
• アッセイは1時間で完了

増殖を測定する迅速かつシンプルな方法
CyQUANT™ NF細胞増殖アッセイでは、細胞溶解、長時間のインキュベーション、放射能、細胞からの染色の除去は不要です。CyQUANT™ NFアッセイでは、細胞透過性のDNA結合色素を細胞膜透過処理試薬と組み合わせて使用することで、オリジナルのCyQUANT™細胞増殖アッセイの凍結融解ステップが不要になります。CyQUANT™ NF細胞増殖アッセイキットは、96ウェルまたは384ウェルのマイクロプレートフォーマットで使用でき、より小さなサンプルサイズ用の200アッセイキット（C35007）と、ハイスループットアプリケーション用の1000アッセイキット（C35006）の2種類の構成で使用できます。

alamarBlue™はTREK Diagnostic Systemsの登録商標です。

研究用にのみ使用できます。診断用には使用いただけません。

仕様

細胞タイプDirect Cell

培養環境懸濁細胞培養、接着細胞培養

検出法蛍光

染色剤タイプその他の標識または色素

励起／発光508/527 nm

フォーマット96ウェルプレート、1536ウェルプレート、384ウェルプレート

インキュベーション時間60分

数量100 Microplates

出荷条件室温

色Green

Emission可視

使用対象（アプリケーション）増殖アッセイ

使用対象 (装置)マイクロプレートリーダー, 分光光度計, 顕微鏡, HTSリーダー

製品ラインCyQUANT、Molecular Probes

製品タイプ細胞増殖アッセイ

Unit SizeEach

組成および保存条件

5 mL CyQUANT™ダイレクト核酸染色（コンポーネントA）
25 mL CyQUANT™ダイレクトバックグラウンドサプレッサーI（コンポーネントB）
どちらも2℃～25℃で保存します。乾燥した状態で、遮光して保護してください。

よくあるご質問（FAQ）

MTT cell proliferation plate assays require cellular metabolism to modify the reagent and thus, only live cells will be counted. Is this true for CyQUANT Direct Cell Proliferation Assay, too?

This is true for MTT (as well as XTT, AlamarBlue Cell Viability Reagent, and PrestoBlue Cell Viability Reagent). CyQUANT Direct will also only count live cells, but for a different reason. The dye in it is a green-fluorescent nucleic acid stain, which will bind to DNA in all cells, live or dead, without the need for cellular metabolism. However, there is a non-cell-permeable quenching reagent in the kit which will both quench extracellular fluorescence (and thus this is a no-wash assay) AND will quench the fluorescence in dead cells (but not live cells).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

MTT cell proliferation assays require cellular metabolism to turn over the reagent, and thus only live cells will be counted. Is this true for CyQUANT Direct as well?

This is true for MTT (as well as XTT, AlamarBlue Cell Viability Reagent, and PrestoBlue Cell Viability Reagent). CyQUANT Direct will also only count live cells, but for a different reason. The dye is a green-fluorescent nucleic acid stain, which will bind to DNA in all cells, live or dead, without the need for cellular metabolism. However, there is a cell impermeant quenching reagent in the kit which will quench both extracellular fluorescence (and thus this is a no-wash assay) and the fluorescence in dead cells (but not live cells).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What volumes should I use to make a 10X stock solution for the CyQUANT Direct Cell Proliferation Assay (Cat. No. C35011, C35012)?

To make a 10X stock solution for the CyQUANT Direct Cell Proliferation Assay (Cat. No. C35011, C35012), we suggest using the following volumes:

1.1 mL PBS
25 µL CyQUANT Direct nucleic acid stain (Component A)
125 µL CyQUANT Direct background suppressor I (Component B)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Are the components of the CyQUANT Direct Red Cell Proliferation Assay and CyQuant Direct Cell Proliferation Assay kits interchangeable?

No. The components of the CyQUANT Direct Red Cell Proliferation Assay and CyQuant Direct Cell Proliferation Assay kits are not interchangeable.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between the CyQUANT Direct Red Cell Proliferation Assay (Cat. Nos. C35013, C35014) and the green fluorescent CyQuant Direct Cell Proliferation Assay (Cat Nos. C35011, C35012)?

The two kits utilize different dyes and background suppressors, but they function in the same way and provide similar results as can be seen from Figures 1 and 2 in the CyQUANT Direct Red Cell Proliferation Assay product sheet (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0018644_CyQUANT_direct_red_assay_PI.pdf). Dyes from both kits are nucleic acid stains and the background suppressors are cell impermeant reagents that quench extracellular fluorescence. They are usable within the same range (100 to 20,000 cells/well in a 96-well format) and in the same workflow.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all CyQUANT™ Cell Proliferation Assays FAQs

引用および参考文献 (17)

引用および参考文献

Abstract

Application of a high-content multiparameter cytotoxicity assay to prioritize compounds based on toxicity potential in humans.

Authors:Abraham VC, Towne DL, Waring JF, Warrior U, Burns DJ,

Journal:J Biomol Screen

PubMed ID:18566484

'Prioritization of compounds based on human hepatotoxicity potential is currently a key unmet need in drug discovery, as it can become a major problem for several lead compounds in later stages of the drug discovery pipeline. The authors report the validation and implementation of a high-content multiparametric cytotoxicity assay based ... More

Activator of G protein signaling 3 promotes epithelial cell proliferation in PKD.

Authors:Nadella R, Blumer JB, Jia G, Kwon M, Akbulut T, Qian F, Sedlic F, Wakatsuki T, Sweeney WE, Wilson PD, Lanier SM, Park F,

Journal:J Am Soc Nephrol

PubMed ID:20488951

'The activation of heterotrimeric G protein signaling is a key feature in the pathophysiology of polycystic kidney diseases (PKD). In this study, we report abnormal overexpression of activator of G protein signaling 3 (AGS3), a receptor-independent regulator of heterotrimeric G proteins, in rodents and humans with both autosomal recessive and ... More

The matricellular protein cysteine-rich protein 61 (CCN1/Cyr61) enhances physiological adaptation of retinal vessels and reduces pathological neovascularization associated with ischemic retinopathy.

Authors:Hasan A, Pokeza N, Shaw L, Lee HS, Lazzaro D, Chintala H, Rosenbaum D, Grant MB, Chaqour B,

Journal:J Biol Chem

PubMed ID:21212276

'Retinal vascular damages are the cardinal hallmarks of retinopathy of prematurity (ROP), a leading cause of vision impairment and blindness in childhood. Both angiogenesis and vasculogenesis are disrupted in the hyperoxia-induced vaso-obliteration phase, and recapitulated, although aberrantly, in the subsequent ischemia-induced neovessel formation phase of ROP. Yet, whereas the histopathological ... More

Selective blockade of cytoskeletal actin remodeling reduces experimental choroidal neovascularization.

Authors:Caballero S, Yang R, Grant MB, Chaqour B,

Journal:Invest Ophthalmol Vis Sci

PubMed ID:21178140

'The efficacy of the peptide Ac-EEED on reducing cell adhesion and proliferation in vitro and choroidal neovascularization (CNV) in vivo was examined. The peptide chimera containing the Ac-EEED sequence was chemically linked to the N terminus of the XMTM delivery peptide from the E(rns) viral surface protein. Ac-EEED or scrambled ... More

Loss of activator of G-protein signaling 3 impairs renal tubular regeneration following acute kidney injury in rodents.

Authors:Regner KR, Nozu K, Lanier SM, Blumer JB, Avner ED, Sweeney WE, Park F,

Journal:FASEB J

PubMed ID:21343176

The intracellular mechanisms underlying renal tubular epithelial cell proliferation and tubular repair following ischemia-reperfusion injury (IRI) remain poorly understood. In this report, we demonstrate that activator of G-protein signaling 3 (AGS3), an unconventional receptor-independent regulator of heterotrimeric G-protein function, influences renal tubular regeneration following IRI. In rat kidneys exposed to ... More

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